What is the principle and working mechanism of the TFF system?

Tangential flow filtration (TFF) is a highly efficient separation technique that can be used to separate, purify, and concentrate biomolecules from complex mixtures. It refers to the filtration form in which the liquid flow direction is perpendicular to the filtration direction.?

? Principles of TFF System:

The TFF system is based on the principle of tangential flow filtration, which is the filtration form in which the liquid flow direction is perpendicular to the filtration direction.?

Traditional filtration methods, such as dead-end filtration, work by forcing the liquid through a filter, causing the buildup of a cake layer or gel layer on the surface of the filter, leading to a decrease in flow rate.?

However, TFF systems work differently, as the liquid is pumped tangentially to the surface of the membrane, producing shear forces that reduce the accumulation of the cake layer and maintain a constant flow rate.

The TFF system uses a semi-permeable membrane with specific pore size to separate molecules or particles from a liquid mixture. When a mixture is introduced into the TFF system, it is passed through the membrane by applying pressure to the liquid.?

The larger molecules or particles in the mixture are retained by the membrane, while the smaller ones pass through to the filtrate.?

? Working Mechanism of TFF System:

The TFF system works in a continuous flow mode, where the feed stream is circulated through the filtration module in a loop.?

The pressure gradient across the membrane generates a shear force that reduces the accumulation of the cake layer on the surface of the membrane, and maintains a constant flow rate.?

The retained molecules or particles are concentrated on the surface of the membrane, while the filtrate containing the smaller molecules or particles is continuously collected.

In conclusion, TFF systems are widely used in the biopharmaceutical industry for the separation and purification of biomolecules from complex mixtures.?

The TFF system operates on the principle of tangential flow filtration, where the liquid flow direction is perpendicular to the filtration direction, reducing the accumulation of cake layer or gel layer on the surface of the membrane.?

Guidling is well-known as one of the leading tangential flow filtration system manufacturers and suppliers in China.

If you're going to wholesale high-quality tangential flow filtration system in stock, welcome contact us to get a free sample from our factory.

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