This Week's Discovery News

This Week's Discovery News

Top Discovery News

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Ileum tissue single-cell mRNA sequencing elucidates the cellular architecture of pathophysiological changes associated with weaning in piglets?

The work has elucidated the single-cell molecular characteristics of the piglet ileum before and after weaning. They have provided an atlas that portrays the landscape of the intestinal pathophysiological inflammatory process associated with weaning, finding a level of conservation between human and pig that support the use of piglets as a model for human infants. (read more)

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Identification of TCR repertoires in asymptomatic COVID-19 patients by single-cell T-cell receptor sequencing

The results revealed significant TCR preference in the AS group, including the skewed usage of TRAV1-2-J33-TRBV6-4-J2-2 and TRAV1-2-J33-TRBV6-1-J2-3. The findings may shed new light on understanding the immunopathogenesis of COVID-19 and help identify optimal TCRs for the development of novel therapeutic strategies against SARS-CoV-2 infection. (read more)

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Single-Cell RNA-Sequencing Reveals the Cellular and Genetic Heterogeneity of Skin Scar to Verify the Therapeutic Effects and Mechanism of Action of Dispel-Scar Ointment in Hypertrophic Scar Inhibition?

The marker genes that were highly expressed in fibroblasts were extracellular matrix (ECM)-related, whereas ECM-receptor interactions and the transforming growth factor (TGF)-β signalling pathway were also found to be active. Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, which was applied to identify the molecular compounds of Dispel-Scar Ointment (DSO), revealed 74 effective chemical components belonging to 14 types of constituents, such as flavonoids, tanshinones, salvianolic acids, glycosides, and phthalides. (read more)

This Weeks Highlighted Technologies

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Single-cell RNA-Seq can be used to examine the expression of individual cells and provides a higher resolution of cellular differences as compared to traditional RNA-Seq. Single-cell RNA-Seq enables us to understand the function of an individual cell in the context of its microenvironment.

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