Video-rate large-scale imaging with Multi-Z confocal microscopy
Volodymyr Nechyporuk-Zloy
10%+ Growth Driver | Manager | Microscopist | DL Imaging
Video-rate large-scale imaging with Multi-Z confocal microscopy
Amaury Badon, Seth Bensussen, Howard J. Gritton, Mehraj R. Awal, Christopher V. Gabel, Xue Han, and Jerome Mertz
https://doi.org/10.1364/OPTICA.6.000389
?Fast, volumetric imaging over large scales has been a long-standing challenge in biological microscopy. To address this challenge, we report an augmented variant of confocal microscopy that uses a series of reflecting pinholes axially distributed in the detection space, such that each pinhole probes a different depth within the sample. We thus obtain simultaneous multiplane imaging without the need for axial scanning. Our microscope technique is versatile and configured here to provide two-color fluorescence imaging with a field of view larger than a millimeter at video rate. Its general applicability is demonstrated with neuronal imaging of both Caenorhabditis elegans and mouse brains in vivo.