Ultra-performance liquid chromatography ACQUITY UPLC H-Class

UPLC vs HPLC

UPLC (Ultra Performance Liquid Chromatography) and HPLC (High Performance Liquid Chromatography) are both liquid chromatography techniques, but they have key differences

• Pressure: UPLC operates at higher pressures (15,000 psi), while HPLC operates at lower pressures (max <5000 psi).
• Particle sizes: UPLC allows for lower particle sizes in columns.
• Applications: UPLC is better for routine analysis with a focus on speed and efficiency, while HPLC remains a robust and reliable option for various applications.

ACQUITY UPLC H-Class

SYSTEM SPECIFICATION

Maximum operating pressure : 15,000 psi

pH range : 1–12.5

Operating flow rate range : Up to 2.2 mL/min

Detector options : PDA, TUV, FLR, RI, ELS, MASS

Maximum samples plates : 96 round-well 350 μL

Operating flow rate range : Up to 2.2 mL/min

External control : Empower Software, MassLynx Software

Pump options : Binary, Quaternary

Solvent conditioning : Active Pre-heating

Column tracking : eCord Technology tracks column information and usage

CALIBRATION

The ability to measure accurately and reliably is to be proved, to get meaningful results. For this, the results of measurement are to be compared with higher standards, for following parameters.

• GPV (Gradient Proportioning Valve) Test
• Flow Rate Accuracy
• Temperature Accuracy Test
• Injector Precision Test
• Injector Linearity
• Injector Carry over
• Detector Linearity Test
• Wavelength Accuracy Test
• Drift and Noise Test

COST FACTOR

ADVANTAGES OF UPLC

Various advantages of UPLC are as follows:

• Require less run time and enhance sensitivity.
• Provides the selectivity, sensitivity, and dynamic range of LC analysis.
• In chromatogram resolved peaks are obtained.
• Speedy analysis, quantify accurately analytes and related products.
• Uses of fine particle (2μm) for packing of stationary phase make analysis fast.
• Time for sample analysis reduced and consumption of solvents is less.
• More products are analyzed with existing resources.
• Increases sample throughput and enables manufacturers to produce more material that consistently meet or exceeds the product specifications, potentially eliminating variability, failed batches, or the need to re-work material.
• Delivers real-time analysis in step with manufacturing processes.

DISADVANTAGES OF UPLC

• Colμmn's lifespan is shortened due to greater pressure, which also requires additional maintenance. Until yet, without the negative effects of high pressure, performances comparable to or even greater have been achieved employing stationary phases with a size of around 2 m.
• Requires additional maintenance. Perform superior analyses without the negative effects of high pressure by using stationary phase with particle sizes of 2 μm

APPLICATIONS

• Pharmaceutical analysis
• Environmental analysis
• Forensic and Toxicological
• Food safety

SUMMARY

UPLC is an advanced Chromatographic technique that gives fast and accurate results. This special technique improves the speed, resolution, and sensitivity of the analysis. The discovery and development of UPLC have opened doors to new insights and uses of chromatography and has reduced the workload and difficulties faced by analysts and scientists.