TRACK: 17 ELECTRON MICROSCOPY
The transmission electron microscope (TEM), scanning electron microscope (SEM), and reflection electron microscope are only a few examples of the various types of electron microscopes (REM.) In this article, each of these varieties of the electron microscope will be discussed in more detail, along with each one’s merits and drawbacks.
The first type of electron microscope, known as a transmission electron microscope, uses a high voltage electron beam to illuminate the material and produce a magnified image of it.
The electron beam is created by an electron gun. The electron beam’s source, a tungsten filament cathode, is often mounted on the gun. The electron beam is focused with the use of electrostatic and electromagnetic lenses and is accelerated by an anode.
The image of the specimen’s microscopic structure created by the electron beam’s scattering as it travels through the sample may be seen through the microscope’s objective lens.
By projecting the image onto a fluorescent zinc sulfide-coated screen, the spatial variation can be investigated. Placing a photographic film into an electron beam to capture the image is yet another way to capture the image. The image can also be shown on a computer screen in real time using a digital camera.
Historically, transmission electron microscope resolution has been constrained by spherical aberration. However, recent advancements have made it possible to get around this problem and boost resolution via hardware spherical aberration correction. As a result, it is now possible to create images with resolutions lower than 0.5 angstroms and magnifications greater than 50 million times.
Raster scanning was a method used by the scanning electron microscope to create enlarged images of the specimen. It focuses an electron beam, which loses energy as it passes through the specimen’s rectangular area. Other types of energy, including heat, light, secondary electrons, and backscattered electrons, are created from the energy. The geography and composition of the original specimen can be seen by translating this data.
Scanner electron microscopes often have lower resolution than a transmission electron microscope.
However, it is advantageous because it makes use of surface processes, which enables it to produce images of huge samples with a wider depth of field and a maximum size of several centimetres. As a result, the images produced by a SEM may be accurate depictions of the specimen’s true shape.
SUB TOPICS:
Electron microscope, tissues, cells, scanning electron microscope, transmission electron microscope, immuno-labeling, Biological, electronic Device, magnified image, biomedical research, organelles, macromolecular, sectioning, immuno-labeling, negative staining, cell disease.??X-rays, immuno-labels, topography, tissue sections, molecules,
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