Tecan Spark? Cyto: Your Plate Reader for Live Cell Imaging and Real-Time Cytometry
Zodel Biotec
A biotechnology company that develops and produce cost effective biotechnology products of international standards.
Tecan Spark? Cyto, the ultimate multi-mode plate reader equipped with fluorescence imaging and cytometry capabilities. This powerful tool is here to transform your cell-based research, offering a fusion of live cell imaging and top-notch detection technologies. With Spark Cyto, you're not just collecting data – you're creating a symphony of qualitative and quantitative insights that redefine your experiments. And the best part? It all happens in real-time, thanks to parallel data acquisition and analysis.
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Why Tecan Spark Cyto?
1. A Deeper Dive into Cell Analysis: Spark Cyto goes above and beyond, merging high-end camera components with innovative patented technology. This synergy enables a comprehensive exploration of your entire cell population. Imagine capturing the entirety of a 96- or 384-well microplate well with a single image. This capability ensures not a single cell escapes your scrutiny. Spark Cyto's three magnification levels and four fluorescence and bright field imaging channels make it a versatile player in cell analysis.
2. Dimensional Data with Every Scan: Spark Cyto comes in customizable configurations, building upon the sturdy foundation of the Spark multimode reader platform. By seamlessly integrating advanced imaging with proven multimode reader abilities, Spark Cyto empowers you to forge new research paths and acquire robust orthogonal data, faster than ever.
3. Boost Your Experiment Throughput: Elevate your experiments with Spark Cyto's seamless integration. Enhance it with an automated multi-plate cell incubator or effortlessly incorporate it into Tecan's automated workflow solutions.
4. Total Experimental Command: Say hello to Spark Cyto's user-friendly design, designed to handle a broad array of common cytometry applications. This means you get a new level of control without sacrificing convenience. With predefined methods for common cytometry tasks, such as image acquisition and analysis, plus features like 'user-defined' parameters and Real Time Experimental Control (REC?), Spark Cyto is your ticket to unlocking innovative applications.
5. Seamless Software Experience: Spark Cyto's hardware prowess is complemented by its software gem – SparkControl. From pre-set methods for common cytometry to personalized protocols and real-time experimental control, this software puts the reins in your hands. The Tecan Connect mobile app ensures you're in the loop even when you're not physically in the lab.
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When Should I Use Tecan Spark Cyto?
You might be wondering when to tap into the magic of Spark Cyto. If your research involves any of the following steps or applications, then Spark Cyto is tailor-made for you:
1. Count Cells, No Dyes Needed: When you need swift and accurate cell counting in bright field images without the fuss of cellular dyes, Spark Cyto's got your back.
2. Visualizing Confluence: Use the bright field imaging channel to quickly gauge cell density in a well. With automated cell confluence calculation and a customizable roughness factor, Spark Cyto simplifies visual confirmation.
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3. Effortless Nuclei Counting: Optimized for Hoechst 33342, Spark Cyto makes cell counting a breeze using blue fluorescent dyes with nuclear DNA binding abilities.
4. Transfection Efficiency Analysis: Spark Cyto's intelligent application calculates transfection rates in cells containing green fluorescent protein (GFP). The green and blue images are overlaid and analysed to measure transfection efficiency.
5. Cell Vitality Assessment: For assessing cell viability, Spark Cyto employs a double staining approach to differentiate between live (green) and dead (red) cells. By utilizing two distinct fluorescent dyes, such as calcein AM for live cells and propidium iodide for dead cells, researchers can swiftly capture images and conduct analyses on their cell population, all within a matter of minutes. This process facilitates a comprehensive understanding of cellular health and viability, making Spark Cyto a powerful asset for time-sensitive experiments.
6. Decoding Cell Death: Spark Cyto can distinguish between apoptosis and necrosis, thanks to differential staining. With proprietary algorithms and markers like Hoechst 33342 and Annexin V-FITC, Spark Cyto lets you dive into cell death mechanisms.
7. Multi-Colour Magic: Whether it's counting cells with multiple labels or markers, Spark Cyto's user-friendly multi-colour application is your go-to tool.
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Other application includes:
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In conclusion, Spark Cyto shines as a versatile gem. Whether you're unravelling cell mysteries at a basic level or delving into specialized applications, Spark Cyto's real-time capabilities, imaging prowess, and user-friendly software make it your trusted companion in the pursuit of scientific knowledge. It's not just about understanding today – it's about exploring tomorrow's discoveries, today.