Single Molecule Delivery into Cells ?? Spatial Transcriptomics at Micron Scale ?? Raman2RNA: Predicting RNA Profiles ??

Single Molecule Delivery into Cells ?? Spatial Transcriptomics at Micron Scale ?? Raman2RNA: Predicting RNA Profiles ??

Bioinformer Weekly Roundup

Stay Updated with the Latest in Bioinformatics!

Issue: 19 | Date: 12 January 2024

?? Welcome to the Bioinformer Weekly Roundup!

In this newsletter, we curate and bring you the most captivating stories, developments, and breakthroughs from the world of bioinformatics. Whether you're a seasoned researcher, a student, or simply curious about the intersection of biology and data science, we've got you covered. Subscribe now to stay ahead in the exciting realm of bioinformatics!

?? Featured Research

Single molecule delivery into living cells | bioRxiv

This study introduces a nanoinjection platform utilising nanopipettes for the precise delivery of macromolecules like DNA and proteins into cells, achieving single molecule resolution and inducing observable phenotypic changes.

TIME-seq reduces time and cost of DNA methylation measurement for epigenetic clock construction | Nature Aging

This research introduces TIME-seq, an efficient and cost-effective method for applying epigenetic 'clocks' based on DNA methylation, demonstrating its accuracy and robustness in various tissues and cells.

Spatial Transcriptomics Sequencing of Mouse Liver at 2 Micron Resolution Using a Novel Spatial DNA Chip | bioRxiv

This study presents a novel high-resolution DNA chip for spatial transcriptomics, offering enhanced mapping of cell types and gene expression patterns in a mouse model.

Unsupervised and supervised discovery of tissue cellular neighborhoods from cell phenotypes | Nature Methods

This paper presents CytoCommunity, an algorithm that identifies tissue cellular neighbourhoods (TCNs) using a graph neural network model, demonstrating its superior ability to discern condition-specific TCNs in spatial omics data.

??? Latest Tools

SPLASH: A statistical, reference-free genomic algorithm unifies biological discovery | Cell

This research introduces SPLASH, a novel genomic analysis method that bypasses reference sequence alignment, enabling the discovery of various types of sequence variations including complex mutation patterns and regulated RNA isoforms.

?? Paper author, Tavor Baharav, has posted a Twitter thread describing the analysis method and implications.

ScGOclust: leveraging gene ontology to compare cell types across distant species using scRNA-seq data | bioRxiv

This study introduces scGOclust, a computational tool leveraging the Gene Ontology knowledge-base to compare cell type expression profiles across species, demonstrating its ability to identify functional similarities and divergences in cell types.

?? Paper author, Yuyao Song, has posted a Twitter thread describing the tool and highlighting its application.

Prediction of single-cell RNA expression profiles in live cells by Raman microscopy with Raman2RNA | Nature Biotechnology

This study introduces Raman2RNA (R2R), a novel method combining hyperspectral Raman microscopy with domain translation to infer single-cell RNA sequencing profiles nondestructively, demonstrating its superiority over brightfield image inferences.

Sopa: a technology-invariant pipeline for analyses of image-based spatial-omics | bioRxiv

This paper presents Sopa, a technology-agnostic and memory-efficient pipeline for analyzing various spatial-omics data, including spatial-transcriptomics and multiplex imaging. Sopa offers a unified platform for tasks like segmentation and spatial analysis, and produces accessible web reports and visualizer files.

scDirect: key transcription factor identification for directing cell state transitions based on single-cell multi-omics data | bioRxiv

This study introduces scDirect, a computational framework designed to identify key transcription factors (TFs) from single-cell multi-omics data, overcoming limitations of traditional methods that rely on cell population data.

?? Community News

Landmark national study supports use of whole genome sequencing in standard cancer care | Genomics England

Landmark national study demonstrates how the integration of health data with whole genome sequence (WGS) data in cancer patients enables doctors to deliver more personalised and effective care.

Prospective study design and data analysis in UK Biobank | Science Translational Medicine

This reports highlights the impact of the UK Biobank's pioneering study design and data access policies on its ability to greatly enhance the capacity for formulating and validating hypotheses concerning the underlying causes of human diseases.

Visium HD Spatial Gene Expression is now available for pre-order | 10x Genomics

Visium HD Spatial Gene Expression is now available for pre-order, offering whole transcriptome spatial gene expression with single-cell scale resolution.

Unveiling Cellular Mysteries: How AI Is Transforming Biological Science | YouTube

This video from CZI showcases how emerging technologies and bioengineering cells are revolutionising our approach to solving complex problems and expanding our knowledge of human biology.

Deep Origin and NVIDIA help researchers save time and money by bringing GPU-accelerated genomics tools to Nextflow | Deep Origin

NVIDIA's Parabricks GPU-accelerated genomics tools have been integrated into Nextflow modules, offering researchers the ability to speed up genomics workloads by up to 100 times.

A society-wide conversation is needed about germline genome editing using CRISPR | Nature Medicine

The potential of therapeutic genome editing to cure rare diseases is immense, yet germline editing currently faces a ban. However, with extensive societal discourse and the backing of national and transnational legislation, there could be a path forward for permitting germline editing under specific circumstances.

Tearing up the traditional biotech playbook | Nature Biotechnology

In today's biotechnology landscape, startups face a challenging funding paradox: they need preliminary data to secure funding, but require funding to generate this data. To move forward, these startups must find creative solutions to overcome the early-stage funding hurdle.

?? Upcoming Events

Spatial Multi-Omics for Cancer Systems Biology, Virtual | 12 February 2024

ISB's free virtual workshop on Spatial Multi-Omics for Cancer Systems Biology includes sessions on experimental and computational methods, practical tutorials, and targets a diverse scientific audience.

Innovations in Single Cell Omics, Barcelona | 27 - 28 May 2024

The ISCO conference focuses on single-cell and spatial technologies, computational methods, and medical applications, offering networking opportunities for PhD students and postdocs.

?? Educational Corner

Housekeeping and Reference Transcript Atlas | HRT Atlas

HRT Atlas v1.0 is a web-based database that provides accurate lists of housekeeping genes and transcripts in humans and mice, utilizing extensive RNA-seq data and offering resources for qPCR experiments and regulatory elements.

Statistical Programming Paradigms and Workflows | Johns Hopkins Bloomberg School of Public Health

This free course covers advanced statistical computing programming paradigms and workflows required for the research and application of statistical methods. The course is developed and maintained by Stephanie Hicks .

Applied Computational Genomics Course | University of Utah

This free course will provide a comprehensive introduction to fundamental concepts and experimental approaches in the analysis and interpretation of experimental genomics data. The course is developed and maintained by Aaron Quinlan .

Finding the right power balance: Better study design and collaboration can reduce dependence on statistical power | PLOS Biology

This essay advocates for a shift away from the dominant focus on statistical power in experiment sample size determination, suggesting a new approach that emphasizes better study designs, (pre)registration, obligatory reporting of all results, team science, multi-institutional collaboration, and prospective and living meta-analyses to enhance the effectiveness and equity of scientific research.

Episode 28: Seqera and Nextflow in public health — Interview with Kelsey Florek | YouTube

In this interview, Rob and Kelsey discuss how genomic surveillance has changed since the COVID pandemic, and how Seqera Platform and Nextflow pipelines have substantially lowered the barrier to entry for bioinformaticians running workflows in public health labs and in the field.

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Disclaimer: The information provided in this newsletter is for educational and informational purposes only and does not constitute professional advice.

Editor: James Ashmore | Contact: [email protected]

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