September 2024: High-content imaging for kidney disease research

Welcome to the September edition of the AI for Live Cell Insights Newsletter, bringing you the latest live cell analyses powering drug discovery and cosmetics development. Each month, we will explore a new application of cellular analysis for label-free live cell imaging, with publication highlights and news from Nanolive SA . This month, we are highlighting the application of Nanolive imaging for kidney disease research, as well as recent publications in the fields of parasitology, immunology and neuroscience, amongst others.

Detecting disease-linked sex differences at the cellular level

Diabetic kidney disease progresses to chronic kidney disease faster in men than women, and for the first time, researchers used Nanolive imaging to detect differences at a cellular level in male and female cells. The group studied the cell surface area and mitochondrial area of male and female human proximal tubular epithelial cells and found significant differences, which correlated to functional assessments of the cells’ glycolysis and oxygen consumption rates. Female cells had a smaller average surface area, as well as lower mitochondrial surface area per cell.

“Sex differences in kidney metabolism may reflect sex-dependent outcomes in human diabetic kidney disease” by Sergi Clotet-Freixas et al. was published in Science Translational Medicine and can be accessed in full here.

Automated analysis of cellular morphology and phenotype

The high resolution of Nanolive’s label-free holotomographic technology makes it possible to image cellular morphology in unprecedented detail. The quantity and complexity of the images generated allows visualization of biological processes from the population to subcellular level. EVE Analytics is an automatic analysis solution to extract meaningful measurements including cell surface area from these high-content images, forming the backbone of Nanolive’s digital assays for phenotypic analysis, as a push-button tool to segment and measure every cell automatically.

Click here to read the eBook for more details about holotomography.

Online webinar

Are you based in the Columbus, Ohio area? Join our specialists in this webinar to discover cell and organelle dynamics live and label-free with Nanolive technology. Holotomography is an innovative imaging technique which combines a rotating light source and the intrinsic refractive properties of cellular structures to produce crystal-clear, high resolution (200 nm) images of cells and their organelles, without labels or damage. Rich image data can be captured and processed automatically with AI-powered analysis to study phenomena such as cellular health, metabolism, trafficking, and phenotypic responses, as well as T cell efficacy and drug delivery. Learn about a platform ideally suited for a wide range of research studies including gene therapy, immuno-oncology, drug development, iPSC differentiation and metabolism.

Sign up here.

Latest publication highlights with Nanolive imaging

·???????? Drug screening: Liao, Y.-C. et al. (2024) ‘Effects of the oxoaporphine alkaloid hernandonine on dengue virus. Evidence for its mechanisms of action’ Phytomedicine https://doi.org/10.1016/j.phymed.2024.155986

·???????? Nanomaterials: Fornasier, M. et al. (2024) ‘Cubosomes and hexosomes stabilized by sorbitan monooleate as biocompatible nanoplatforms against skin metastatic human melanoma’ Journal of Colloid and Interface Science https://doi.org/10.1016/j.jcis.2024.08.126

·???????? Mitochondria: Yang, C. et al. (2024) ‘Pulsed electromagnetic fields regulate metabolic reprogramming and mitochondrial fission in endothelial cells for angiogenesis’ Scientific Reports https://doi.org/10.1038/s41598-024-69862-x

·???????? Microbiology: Lei, Q. et al. (2024) ‘Bioprocessing strategies for enhanced probiotic extracellular vesicle production: culture condition modulation’ Frontiers in Bioengineering and Biotechnology https://doi.org/10.3389/fbioe.2024.1441552

·???????? Neuroscience: Tao, T. et al. (2024) ‘Research of Brain Targeting Nano?Metformin on Cerebral Ischemia-Reperfusion Injury Based on Microglial Cell Activation’ SSRN https://dx.doi.org/10.2139/ssrn.4717704

·???????? Parasitology: Larrazabal, C. et al. (2024) ‘Besnoitia besnoiti tachyzoite replication in bovine primary endothelial cells relies on host Niemann–Pick type C protein 1 for cholesterol acquisition’, Frontiers in Veterinary Science https://doi.org/10.3389/fvets.2024.1454855

·???????? Plant physiology: Landes, T. et al. (2024) ‘Brillouin light scattering microscopy reveals micro-mechanical inhomogeneities of the apple fruit cuticle’ Research Square https://doi.org/10.21203/rs.3.rs-4606111/v1

·???????? Parasitology: Renteria-Solis, Z. et al. (2024) ‘Interaction of Chicken Heterophils and Eimeria tenella Results in Different Phenotypes of Heterophil Extracellular Traps (HETs)’, Poultry https://doi.org/10.3390/poultry3030024

·???????? Neutrophil extracellular traps: Singh, J. et al. (2024) ‘Calpain-1 weakens the nuclear envelope and promotes the release of neutrophil extracellular traps’, Cell Communication and Signaling https://doi.org/10.1186/s12964-024-01785-6

Find over 250 publications featuring Nanolive imaging here.

Online demo

Find out how the Nanolive imaging system works in our online demo of the Nanolive imaging platform and software. You can watch on-demand here.

Subscribe to this monthly newsletter to stay up to date with AI applications in cell biology by clicking the button at the bottom of the page.