SEC-HPLC to monitor AAV8 and AAV9
Citation Bee, Jared S., et al. "Mechanistic Studies and Formulation Mitigations of Adeno-Associated Virus Capsid Rupture during Freezing and Thawing: Mechanisms of Freeze/Thaw Induced AAV Rupture." Journal of Pharmaceutical Sciences (2022).

SEC-HPLC to monitor AAV8 and AAV9

Comments: We are fortunate, as one of the authors, @Jared Bee has graciously shared a link to download this reference for free for the next 50 days! Please give credit to his recent post, where the journal link can be accessed free of charge -

https://www.dhirubhai.net/feed/update/urn:li:activity:6918533773273743360/

Also please check out the comments section about the impact of P188, has anyone else used this in their formulations for AAV? #aav #viralvectors #genetherapy #formulationdevelopment #freeze #stability

Analytical SEC HPLC of AAV8 and AAV9 with Sepax SRT SEC-1000 PEEK

Mechanistic Studies and Formulation Mitigations of Adeno-associated Virus Capsid Rupture During Freezing and Thawing: Mechanisms of Freeze/Thaw Induced AAV Rupture


Company Name:?REGENXBIO Inc.

Year:?2022

Product:?Sepax SRT SEC-1000 PEEK

Part Number:?215950P-4630

Separation Mode:?SEC

Broad Sample Category:?VLP/Virus/Vaccine

Specific Sample:?AAV8 and AAV9

Mobile Phase:?20 mM sodium phosphate, 300 mM sodium chloride, 0.005% w/v poloxamer 188, pH 6.5

Instrument:?UV

Full Title:?Mechanistic Studies and Formulation Mitigations of Adeno-associated Virus Capsid Rupture During Freezing and Thawing: Mechanisms of Freeze/Thaw Induced AAV Rupture

Citation:?Bee, Jared S., et al. "Mechanistic Studies and Formulation Mitigations of Adeno-Associated Virus Capsid Rupture during Freezing and Thawing: Mechanisms of Freeze/Thaw Induced AAV Rupture." Journal of Pharmaceutical Sciences (2022).

Link to Paper:?https://www.sciencedirect.com/science/article/pii/S0022354922001265?dgcid=author

Thank you to the authors and supporting scientists!

Jared Bee

Head of Formulation and Drug Product Development

2 年

Thanks for the shout out to my analytical colleagues for this method. The results shown here are for ‘F4’ which was optimized to have low rupture during freeze/thaw - the column/method worked really well to show a much greater progressive increase in a free DNA pre-peak in F1 and F2 subjected to freeze/thaw (available in supplemental data of referenced paper).

要查看或添加评论,请登录

Annie Biggs Sanger的更多文章

  • SEC of circRNA

    SEC of circRNA

    ***Special callout as one of the lead researchers who performed this work, Piotr Kowalski, has an open position at his…

    1 条评论
  • Purification of Cas9 with SRT-10 SEC 300

    Purification of Cas9 with SRT-10 SEC 300

    ***Special callout as the chromatographer who performed this work, William Selleck, is available for consulting work!…

    2 条评论
  • SEC-SWATH-MS

    SEC-SWATH-MS

    Explain Like I'm Five | ELI5 To my friends and family not in the biopharma industry: Envision possessing a sizable…

    2 条评论
  • Analytical SEC of an ADC

    Analytical SEC of an ADC

    Hiding Payload Inside the IgG Fc Cavity Significantly Enhances the Therapeutic Index of Antibody?Drug Conjugates…

    1 条评论
  • AEX-MS of HSA isoforms

    AEX-MS of HSA isoforms

    Explain Like I'm Five | ELI5 To my friends and family not in the biopharma industry: Albumin is a protein found in our…

  • Determination of residual dextran sulfate in protein products by SEC–HPLC

    Determination of residual dextran sulfate in protein products by SEC–HPLC

    Explain Like I'm Five | ELI5 To my friends and family not in the biopharma industry, dextran sulfate is a special kind…

  • Selection and Purification of Sybodies

    Selection and Purification of Sybodies

    In vitro and ex vivo proteomics of Mycobacterium marinum biofilms and the development of biofilm-binding synthetic…

  • Purification of circular RNA

    Purification of circular RNA

    RNA Circularization: Circular RNAs (circRNAs) are single-stranded RNAs with a contiguous structure that have enhanced…

  • Chats with openAI - Chromatography Edition

    Chats with openAI - Chromatography Edition

    Don't yet put your faith in AI for your #analyticaldevelopment needs. (Annie) what is the estimated hydrodynamic side…

    2 条评论
  • The Complexities of Developing Therapeutic Antibodies: Navigating the Minefield of Non-Specificity

    The Complexities of Developing Therapeutic Antibodies: Navigating the Minefield of Non-Specificity

    Antibodies, highly potent therapeutic scaffolds, compose over 100+ approved products on the market. The development of…

    3 条评论

社区洞察

其他会员也浏览了