Nucleic Acid Insights

Accelerating #LNPoptimization and development Optimizing #lipidnanoparticle (#LNP) manufacturing processes manually is challenging, and switching equipment for clinical production requires re-optimization, which takes a lot of time and resources. Next month, join Unchained Labs’ Kevin Lance (Director of Marketing) as he introduces a combination of novel #LNPproduction and analysis tools, offering continuous production and #rapidparticleanalysis, significantly reducing the workload for encapsulation efficiency #assays Register for this upcoming #webinar below:

#Interview now available! From bench to bedside: advancing #mRNAtherapies for #cardiovasculardiseases The #mRNAtherapeutics field is heavily engaged in extending its reach to new cells, targets, and patient populations, and the opportunities don’t come any bigger than those in the cardiovascular diseases area. Here, Ajit Magadum (Assistant Professor, Department of Cardiovascular Sciences and Aging and Cardiovascular Discovery Center (ACDC), Lewis Katz School of Medicine at Temple University) talks about his work in identifying novel targets and addressing #mRNAtargeting specificity to improve patient outcomes. Read at your convenience below:

Session 2: Enhancing #mRNAproduction with #advancedanalytics mRNA production involves complex #IVT mixtures, including #targetmolecules, process- and product-related impurities - all with similar biophysical characteristics. Designing a correct hardware setup for reproducible analytical separation of these large biomolecules is a significant challenge. Next month, Michael Daume (WACKER Biotech) is joined by Blaz Bakalar (Sartorius BIA Separations), as he explores innovative solutions for streamlining mRNA production. This session forms part of a #virtualconference, where biopharmaceutical industry leaders and experts will share insights and success stories in applying novel analytical systems for large molecules. Attend this event to: ? Understand the complexities of mRNA production and the challenges associated with separating large biomolecules. ? Learn how to accelerate your process development workflows with rapid feedback ? Participate in an interactive Q&A session to get your questions answered and gain deeper insights into improving your #mRNA production. To register & view the full agenda below: https://buff.ly/4cWlC8N

#Article now available! Process optimization of #mRNApurification for #vaccines and therapeutic applications Critical to bringing #mRNAtherapeutics to patients is an efficient, effective, and scalable #downstreamproduction process. This article explores the development, characterization, and optimization of an #mRNA capture step using POROS? #Oligo (dT)25 #AffinityResin. This resin provides an #affinitysolution that leverages the rigid POROS backbone with its linear pressure-flow relationship, enabling a consistent purification platform from process development through to clinical manufacturing. Read below:

Engineering lipid nanoparticles for #lungcancerimaging Early detection of lung cancer significantly improves survival rates, yet only 27% of all lung cancers are diagnosed at Stages 1-2. This is partly due to the lack of highly accurate, non-invasive diagnostic imaging methods that can differentiate malignant cancers from benign lesions. Next month, join Blaine McCarthy (Team Lead, Delivery, Earli Inc.), Sushil Lathwal (Senior Scientist, Earli Inc.), and Ben Knappett (Market Manager of LNPs, Unchained Labs) as they introduce the workflow used to engineer #lipidnanoparticles (#LNPs) to deliver cancer-activated #DNAvectors that produce synthetic biomarkers for #cancerimaging. Register for this #webinar below:

Precise #mRNApurity assessment for accelerated therapeutic development and #releasetesting The quantification of #mRNA in solutions presents an array of challenges, including a lack of accuracy when determining purity ratio with traditional technologies, and the introduction of potential errors and risks through sample preparation or dilution. Next month, Repligen Corporation's Paul Mania (Bioanalytics Applications Specialist) will introduce a precise #mRNApurity assessment technique that provides results that closely match theoretical purity ratios while avoiding risks associated with sample dilution, enhancing the efficiency of #mRNAproduction. Register for this #webinar below:

Implementation of an improved platform for downstream processing of #plasmidDNA pDNA represents the backbone of most cell and #genetherapy production workflows. As a key raw material, it can have an important effect on subsequent downstream processes, therefore it is crucial to implement strategies that maximize its purity and yield. Next month, AstraZeneca’s Justina Martinkiene (Associate Scientist, Purification Sciences) and Sartorius BIA Separations’ Klemen Bo?i? (Process Development Scientist) will provide insights on how to optimize the #pDNA capture process, and showcase data on a novel approach for removing undesired #pDNAisoforms. Register for next month’s #webinar below:

Session 1: Enhancing #pDNA production for #genetherapyManufacturing pDNA and #mRNA, as well as characterizing delivery systems like #LNPs, is a complex process that demands innovative analytical methods to improve yield and purity. Next month, join Ahmet Hilmi Tekin (BioNTech SE) and Blaz Bakalar (Sartorius BIA Separations) as they share their struggles and successes of establishing a pDNA manufacturing platform. This session forms part of a virtual conference, where biopharmaceutical industry leaders and experts will share insights and success stories in applying novel analytical systems for large molecules. Attend this event to learn about: ? The latest trends and challenges in pDNA and mRNA production ? Case studies demonstrating innovative analytical solutions for reliable in-process control in pDNA and mRNA process development ? The key parameters for #characterizingLNPs, and the challenges associated with traditional analytical methods ? Enhancing the safety and reliability of LNP formulations by providing comprehensive insights into their development and optimization Register & view the full agenda below: https://buff.ly/4cWlC8N

#Downstreamprocessing in the #nucleicacids field: optimizing purification of #mRNA and #pDNA With the search for efficient, consistent, and scalable #bioprocesssolutions for #RNA and #DNA continuing to intensify, adaptable downstream processing tools and platforms have come to be of central importance. In this #eBook, experts present in-depth technical information and practical advice to identify and develop optimized and future-proofed downstream process workflows for both mRNA and pDNA Read below:

Unlocking #mRNA-#LNP potential using a novel lipid library combined with innovative #analyticalmethods #Lipidnanoparticles (LNPs) have seen success in their use for #RNAdelivery in various therapies. However, LNPs still exhibit limitations including a tendency to accumulate in the liver and strict requirements for post-formulation quality monitoring. Next week, Polyplus now part of Sartorius’ Guillaume Freund (Process Technology Manager) and Sartorius BIA Separations’ Nejc Pavlin (Process Analytics Development Manager) will discuss novel strategies for mRNA-LNP development such as a library of imidazolium-based lipids and liquid #chromatography to ensure the quality, efficacy, and safety of LNPs in various applications. Register for this upcoming #webinar below: