ProMetic's Range of Ion Exchange Resins

Ion exchange (IEX) chromatography is used to separate proteins on the basis of their net charge at a particular pH. A protein is positively charged when the pH is below the pI of the protein and is negatively charged when the pH is above the PI of the protein.

IEX chromatography separates proteins by a reversible interaction between the charged protein and an oppositely charged adsorbent. Typically, binding is performed in a low salt buffer and elution is achieved by the addition of salt and/or adjustment of pH using either a step-wise or a gradient elution.

Anion exchangers (positively charged adsorbents) are used when the protein(s) to be bound are negatively charged, whereas cation exchangers (negatively charged adsorbents) are used when the protein(s) to be bound are positively charged.

Ion exchangers are categorized as either strong or weak ion exchangers, which is defined by the pH range over which the adsorbent remains charged, with strong ion exchangers being charged over a wider pH range than weak ion exchangers.

ProMetic BioSciences Ltd (PBL) has developed four IEX adsorbents, which have broad applicability: DEAE PuraBead? HF (weak anion exchanger); Q PuraBead? HF (strong anion exchanger); CM PuraBead? HF (weak cation exchanger) and SP PuraBead? HF (strong cation exchanger).

PBL’s ion exchange products are agarose-based chromatography adsorbents with excellent flow properties and high binding capacities. They have been designed for capture, intermediate purification and polishing applications andengineered for use in demanding process applications. They benefit from PBL’s proprietary PuraBead? 6HF base matrix technology with a near mono-disperse particle size distribution, which provides high resolution, reduces the risk of column fouling and renders the adsorbents easy to pack.

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