Probiotics for lowering of cholesterol

Probiotics for lowering of cholesterol

There have been several mechanisms proposed for the purported cholesterol lowering effects of probiotics, including deconjugation of bile acids by bile-salt hydrolase enzymes of probiotics assimilation of cholesterol by probiotics co-precipitation of cholesterol with deconjugated bile cholesterol binding to cell walls of probiotics incorporation of cholesterol into the cellular membranes of probiotics during growth , conversion of cholesterol into coprostanol and productionof short-chain fatty acids upon fermentation by probiotics in the presence of prebiotics.

 

Numerous studies on the effects of probiotics on cholesterol have used animals as models. Shinnick et aL.  studied the effect of processing on the ability of oat fiber to lower plasma and liver cholesterol concentrations in rats. Rats were fed diets containing 6% dietary fiber as cellulose, oat bran, high fiber oat flour or one of four processed high fiber oat flours for 3 wk; all diets also contained 1.0% cholesterol and 0.2% cholic acid. All of the oat products significantly lowered plasma and liver cholesterol without depressing food intake or weight gain. A study was conducted to determine the effect of glucomannan, chitosan), or an equal mixture of the two on cholesterol absorption and fat and bile acid excretion in rats .Total liver cholesterol was significantly reduced in all groups compared with the control group. Cholesterol absorption was significantly reduced from 37.5% in the control group to 20.2% in the glucomannan group, 18.2% in the combination and 9.4% in chitosan. Mice deficient in the LDL receptor have also been widely used as a model to mimic human atherosclerosis by feeding them Western diets with or without insulin-sensitizing agents; however, the time-course of atherosclerotic lesion development and distribution of lesions at specific time-points are yet to be established .Lin et aL.  conducted a study aimed to investigate whether the combination of plant sterol esters with soy protein or soy isoflavones would have extra cholesterol-lowering effects. Hamsters were fed diets containing casein (control), plant sterol esters, intact soy protein, soy isoflavones, plant sterol esters plus soy protein, or plant sterol esters plus soy isoflavones for 5 wk. All diets contained 0.08 g cholesterol/100 g diet. The plant sterol esters and the soy protein diets significantly lowered the plasma total cholesterol concentration by 13% and 9%, respectively as compared to the control, whereas the isoflavone diet had no effect. The combination of plant sterol esters and soy protein decreased plasma total cholesterol by 26%. In conclusion, the combination of PSE and soy protein more dramatically lowers plasma lipids than the individual ingredients. Guinea pigs  and pigs  have also been used as models for humans in studies of cholesterol lowering drugs. Because these animals share some attributes with humans in terms of cholesterol and bile acid metabolism, plasma lipoprotein distribution, and regulation of liver cholesterol enzymes  and digestive anatomy and physiology ] they are useful experimental models for research. Therefore the cholesterol lowering effects seen in these animal studies suggest a similar potential in humans.

Although numerous studies have demonstrated convincing cholesterol-lowering effects of probiotics in both animals and humans, controversial results have surfaced. Hatakka et aL. reported that the administration of L. rhamnosus (1010 CFU/g per capsule; two capsules daily) for 4 weeks did not influence blood lipid profiles in thirty-eight men with mean cholesterol levels of 6.2 mmol/L (240 mg/dL). In another study involving forty-six volunteers (aged 30 to75 years old), Simons et aL. found that the consumption of Lactobacillus fermentum, (2×109 CFU per capsule; four capsules daily) did not result in any lipid profile changes after 10 weeks. Lewis and Burmeister  conducted a randomized, placebo-controlled double blind and crossover designed study to determine the effect of Lactobacillus acidophilus on human lipid profiles. In the study, eighty volunteers 20to 65 years of age with a baseline total cholesterol of more than 5.0 mmol/L (greater than 193 mg/dL) and a mean Body Mass Index of 27.8 kg/m2 consumed two capsules containing freeze-dried L. acidophilus (3×1010 CFU/2 capsules) three times daily for six weeks, and crossed over for another six weeks after a 6-week washout period. The authors found that L. acidophilus capsules did not significantly change plasma total cholesterol, LDL-cholesterol, HDL-cholesterol and triglycerides of the subjects.

 

Even in vivo trials using human subjects are also affected by external factors such as experimental design, lack of adequate sample size for statistical purposes, failure to control for other nutrient intake and energy expenditure during the experiments, and different strains and dosages of probiotics . There is a need for large-scale clinical trials with controlled dosing, determination of viability, definition of strains and other critical variables to provide the necessary scientific evidence required to determine efficacy of the ever-increasing use of probiotics.

Sonali Patnaik

Anthropologist, Gender & Inclusion Practitioner, Socio-Economic & Environment Change and Transformation Enabler, Social Protection, Safeguards, Livelihoods, PR SEAH, Board Adviser, Social Entrepreneur,

8 年

Hey Sidhartha ! Good to see you on LinkedIn . Very interesting write up. Thanks for sharing.

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