Polyphenol Clean-up with CrossTOX?

Polyphenols are often used as food supplements, but also for flavouring food. Their use is subject to mandatory declaration. Therefore, the examination for polyphenols is an important aspect in connection with "food fraud" and food deception.

Example:

The polyphenol pattern in wine depends, among other things, on the type of maturation. For example, storage in wooden barrels produces a different, more valuable aroma than storage in tanks. Due to the high costs of barrel ageing, falsely declared wines are repeatedly put on the market. This intentionally deceives the customer in order to achieve a higher sales price. In 2021, 1.7 million litres of falsely declared alcohol were unmasked in Europe (source European Anti-Fraud Office (OLAF).

Differentiated and selective polyphenol determinations usually require a lot of experimental work and time. These investigations can be?significantly simplified?by the CrossTOX? clean-up column. Faster, cleaner?sample preparation with concentration of the analytes allows?reliable?determination of polyphenols, e.g. in LC-MS/MS?without contamination?of the analytical instrument by matrix components.

LCTech, in collaboration with CVUA Rheinland, has developed a clean-up to bind specific phenolic compounds to SPE cartridges to reduce matrix compounds that could negatively affect analytical results.

The exact method or the complete application note?"Polyphenol Clean-up with CrossTOX?. Food Fraud / Food Authenticity"?with results can be found?here.

Conclusion:

The CrossTOX? cartridge is ideally suited for the selective concentration of polyphenols. Spirits flavoured with polyphenols can be identified as suspicious by the analysis and ratio determination of individual indicators (e.g. vanillin, syringaldehyde), after cleaning with CrossTOX? SPE cartridges and LC-MS/MS analysis.