Observation on the repair effect of sodium butyrate on intestinal mucosa of broilers infected with E.tenella
Ⅰ Test purpose: to study the effect of sodium butyrate on intestinal mucosa of broilers infected with E.tenella.00
Ⅱ Test material: Avian broiler, Eimeria tenella virulent strain, Sodium butyrate, Coccidiosis drug (sulfachloropyrazine sodium soluble powder).
Ⅲ Test method：
1. Test grouping: Chickens with healthy body weight difference of 10 g were selected. Randomly divided into twelve groups, half male and half female. The weight of each chicken was weighed, and 0.5×104 and 1.0×104 sporulated oocysts of E. tenella were infected orally according to the experimental grouping.
The animals were raised in a flat way, and the feeding management was carried out according to the routine immunization procedure. See Table 1 for detailed data.
2. Measurement indicators and methods:
Growth performance: calculating FCR.
Fecal score: refer to the standard of Morehouse(1970).
Cecal lesions score: according to Johnson and Reid(1970).
Mortality.
Changes of intestinal mucosa, determination of intestinal wall thickness and villus length: Five broilers were dissected in each group on the 7th day (14th day) after infection. After that, every 7-10 days, 5 broilers in each group were dissected until they were reared for about 52 days. Tissue sections were stained by HE method, and the pathological changes of intestinal tract and intestinal mucosa after coccidiosis infection were observed and photographed under microscope. Intestinal wall thickness and villus length were observed and measured under optical microscope.
Ⅳ Result and Analysis:
1. The effect of sodium butyrate on FCR of broilers in each group is shown in table 2.
Table 2 Effect of sodium butyrate on FCR of broilers
Eimeria tenella mainly damages the intestinal mucosa, which affects the absorption of nutrients and increases the FCR. It can be seen from Table 2 that sodium butyrate can alleviate the gastrointestinal injury caused by coccidiosis, and 0.2% sodium butyrate is more effective than 0.1%. The synergistic effect of sodium butyrate and anticoccidial drugs is more obvious than that of sodium butyrate alone.
2. The fecal score in each group is shown in table 3.
Table 3 The fecal score in each group
3. The cecal lesions of broilers is shown in table 4.
Table 4 Cecal lesions in each group
4. Mortality of broilers is shown in table 5.
Tabel 5 Mortality of broilers in each group
Adding sodium butyrate to the diet of broilers infected with E.tenella can alleviate cecal lesions and reduce the discharge of blood and feces. Sodium butyrate, as a rapid energy source of intestinal mucosal epithelial cells, promotes the proliferation and maturation of intestinal epithelial cells, maintains the structural integrity of intestinal mucosal epithelial cells, maintains the normal morphology of intestinal mucosal epithelial cells, maintains the normal mechanical barrier function of intestinal mucosa, and plays a rapid role in repairing intestinal mucosa.
Therefore, adding sodium butyrate in diet can repair intestinal mucosa, reduce cecal lesions and reduce the mortality of chickens.
5. The effect of sodium butyrate on intestinal villi and intestinal wall thickness is shown in Table 6.
Table 6 Intestinal villi and intestinal wall of broilers in each group
Sodium butyrate can promote the proliferation of intestinal mucosal epithelial cells and nourish intestinal villus epithelial cells.
1. Effect of sodium butyrate on intestinal histological changes of broilers infected with E.tenella
E.tenella is mainly parasitic on the cecum and its adjacent intestinal tissues, so the main lesions are also concentrated in the cecum. At the age of 14 days, except the no-treatment control group, all the other groups had different degrees of lesions. At the age of 24 days, the damaged parts of duodenum, jejunum and ileum were basically repaired, and the seriously damaged parts were replaced by hyperplastic muscle tissue.
Tabel 7 Effect of sodium butyrate on intestinal histological changes of broilers
