Nucleic Acids and Protein Calculation Conversions

1. Nucleic Acids

2. Protein

3. Nucleic Acids ←→ Protein

4. Extinction coefficient of deoxyribonucleoside triphosphate (dNTP) (pH7.0)

5. Extinction coefficient of ribonucleoside triphosphate (NTP) (pH7.0)

6. Formulas for DNA Molar Conversions

To calculate the specific values of oligonucleotides, use the extinction coefficients of the various bases and calculate according to the following formulas.

7. Spectrophotometric Conversions

1 A260 unit dsDNA=50 μg/ml

1 A260 unit ssDNA=33 μg/ml

1 A260 unit ssRNA=40 μg/ml

8. Protein Molar Conversions

100 pmol of 100 kDa protein molecule = 10 μg

100 pmol of 50 kDa protein molecule = 5 μg

100 pmol of 10 kDa protein molecule = 1 μg

100 pmol of 1 kDa protein molecule = 100 ng

9. DNA Molar Conversions

1 μg of 1000 bp DNA = 1.52 pmol (3.03 pmol of ends)

1 μg of pBR322 DNA = 0.36 pmol DNA 1 pmol of 1000 bp DNA = 0.66 μg 1 pmol of pBR322 DNA = 2.8 μg

10. Protein/DNA Conversions

1 kb DNA can encode 333 amino acids = 37 kDa protein molecule

270 bp DNA = 10 kDa protein

9 amino acids = 1 kDa protein molecule

810 bp DNA = 30 kDa protein molecule

2.7 kb DNA = 100 kDa protein molecule

11. Formulas for DNA Molar Conversions

12. lsotope Data,Acids & Bases, Protein Data

Isotope

13. Acids and bases

NameFormulaMolecular weightDensityMass percentC molar concentrationGlacial

14. Protein

Strain: E. coli or Salmonella typhimurium

15. Nucleotide Physical properties

16. Nucleic acid data

The average DNA molecular weight of a base pair (sodium salt) =650 Dalton

1.0 A260 unit dsDNA= 50 μg/ml = 0.15mM (in nucleotides)

1.0 A260 unit ssDNA= 33μg /ml= 0.10mM (in nucleotides)

1.0 A260 unit ssRNA= 40μg /ml= 0.11mM (in nucleotides)

dsDNA molecular weight (Dalton)= the number of base pairs ×650

Number of dsDNA molecule terminal moles=2×DNA mass(g) /DNA molecular weight (Dalton)

Number of DNA terminal moles after restriction endonuclease digestion:

a) Circular DNA molecule :2× number of DNA moles × number of bits

b) Linear DNA molecules :2× number of DNA moles × number of bits +2×number of DNA moles

1 μg 1000 bp DNA= 1.52 pmol = 9.1 × 1011 molecules

1 μg pUC18/19 DNA (2686 bp) = 0.57 pmol= 3.4× 1011 molecules

1 μg pBR322 DNA (4361 bp) = 0.35 pmol = 2.1 ×1011 molecules

1 μg M13mp18/19 DNA (7249 bp) = 0.21 pmol = 1.3× 1011 Molecules

1 μg λDNA (48502 bp) = 0.03 pmol = 1.8×1010 molecules

1 pmol 1000 bp DNA = 0.66μg

1 pmol pUC18/19 DNA (2686 bp) = 1.77μg

1 pmol pBR322 DNA (4361 bp) = 2.88μg

1 pmol M13mp18/19 DNA (7249 bp) = 4.78μg

1 pmol λDNA (48502 bp) = 32.01μg

1.0 kb DNA= the coding amount of 333 amino acids ≈37,000 Dalton protein

10,000 Dalton protein ≈270 bp DNA

50,000 Dalton protein ≈1.35 kb DNA