Novel Enzymes to Solve Science’s Most Challenging Problems

The biotechnology and pharmaceutical industries are constantly seeking novel enzymes for active pharmaceutical ingredient (API) synthesis, to replace complex chemical reactions with biocatalysts and to solve their most challenging scientific problems. Enzymes are the fundamental tools driving innovative solutions for a sustainable scientific future in biotech, cosmetics, functional foods, and numerous other areas.

Biosynth's enzyme division has established itself as a leader in manufacturing innovative enzymes for such applications. Our enzyme portfolio has been expertly put together to solve the challenges faced by the modern scientific field. We strive to meet our customer's needs, supplying enzymes with chiral- and regioselectivity, broader ranges of temperature, and pH profiles, among other desired properties, making them suitable across various disciplines. Below we will highlight a handful of Biosynth's novel research enzymes and how they are helping to solve science's most challenging problems.

Glucosyltransferases for Custom Glucoside Synthesis

Glucosyltransferases (GTases), transfer glucose to another organic molecule (aglycon) establishing glycosidic linkages in a process termed glycosylation.?Glucosyltransferases exhibit remarkable substrate specificity, recognizing specific sugar residues and acceptor molecules, which allows for the precise control of glycosylation patterns in cells.

Using glucosyltransferases to add a sugar moiety to a product increases its hydrophilicity, a sort after activity for water-soluble formulations in the cosmetic industry. Not only this but glucosyltransferases can be utilized to synthesize aliphatic, terpene, aromatic, and heterocyclic glucosides, used in flavor or fragrance applications. In the pharma industry, glycosylating active pharmaceutical ingredients can improve the solubility of active ingredients, giving patients the option to take medication in a liquid form.

The natural use of glucosyltransferases in plants illustrates how these enzymes can be applied to cosmetics and industrial safety. Plants produce volatile metabolites during the day to deter predators and attract pollinators. At night, these smells are "turned off" by attaching a glucose molecule via glucosyltransferase, increasing solubility and stability for storage. This process can enhance industrial safety using the following novel technology. An odor-producing molecule is glycosylated and immobilized on a sticker for industrial equipment. At normal temperatures, the molecule remains stable, but if equipment overheats, the bond breaks, releasing an unpleasant smell to warn operators. This system offers an advantage over digital systems that can fail during power outages or breakdowns.

Download our glucosyltransferase flyer

Biosynth has added a new collection of diverse glucosyltransferases (GTases) to our catalog.?Our glucosyltransferase kit contains 8 selected, lyophilized enzymes, covering a wide range of different aglycons for the synthesis of glucosides: aliphatic, terpene, aromatic, and?heterocyclic glucosides. Perfect for pharma and cosmetic applications, Biosynth's custom enzyme development suite now offers custom glucoside synthesis based on our full library of over 70 different glucosyltransferases.?Find out more on our glucosyltransferase webpage.

An Alarming Problem Solved by Catalase

Hygiene testing is crucial for hospitals and industries such as food and pharma, who use hydrogen peroxide to disinfect manufacturing equipment and surfaces. Hydrogen peroxide is highly advantageous for this use due to its universal applicability, simple handling, virtually no residues, and finally its lack of environmental toxicity (due to its complete breakdown into oxygen and water). In these types of sterile facilities, it is common practice to use growth media to sample surfaces for remaining contaminants. However, traces of residual hydrogen peroxide can prevent microorganism growth in the plates, leaving the risk of false negative results and a false sense of security. Such environments do not have the luxury of time and cannot simply wait for hydrogen peroxide to degrade. A solution to this problem is imperative.

Biosynth’s ECAT01? Catalase

The addition of catalase ECAT01? to agar plates removes remaining traces and spots of high concentrations of hydrogen peroxide. This offers a more effective and reliable solution for safe and secure environmental monitoring of sterilization in clean rooms, isolators, or production facilities.

Biosynth’s innovative catalase ECAT01? is potent, highly stable, and effective in growth media applications. Even at temperatures below 50°C, in growth media without agar, and in those that are gamma irradiated.

If you are a manufacturer of contact plates, working in hygiene testing, or a scientist in need of a way to test the sterility of equipment and surfaces rapidly and reliably, add Biosynth’s ECAT01? catalase to your growth media. With our catalase, we can guarantee safe environmental monitoring for your application. That is why we also offer contact plates readily supplemented with our catalase. Contact us for more information.

A Reliable Supply of the 'Almost' Commercially Unattainable Phospholipase D

Biosynth offers a unique Phospholipase D 040 Halal and Kosher Food grade bulk enzyme. With one of the most competitive activity rates on the global market and manufactured in Europe, this enzyme is perfect for use in food, diagnostic, therapeutic, and nutraceutical industries worldwide.

Our enzyme can be harnessed to synthesize critical raw materials such as phosphatidylserine, an important functional ingredient in reducing cognitive dysfunction and dementia in the field of nutraceuticals. As a Halal and Kosher food-grade enzyme, Biosynth's Phospholipase D is an excellent candidate for a variety of food industry applications. Furthermore, our enzyme can be used in diagnostic assays in the quantification of blood phospholipids, creating first-class drug delivery systems and APIs.

We also allow customers to screen for their ideal Phospholipase enzyme using our Phospholipase D Kit which contains 4 highly selective, recombinant phospholipase D enzymes with a broad pH range for transphosphatidylation reactions (converting phospholipids into functionalized lipids for application in API synthesis).?Read?more in our Phospholipase D blog.

Lipases

Sitting under the esterase umbrella are the versatile enzymes, known as lipases. Famous for the hydrolysis of ester bonds in triglycerides, producing free fatty acids and glycerol, lipases demonstrate remarkable catalytic capabilities and serve many critical processes in most organisms. This is clear from their role in the processing, digestion, and transport of lipids and their ability to carry out several bioconversion reactions including?esterification, interesterification, aminolysis, hydrolysis, alcoholysis, and acidolysis. In cosmetics and pharma, these enzymes are involved in generating esters for fragrance and the chiral resolution, regioselective hydrolysis, and synthesis of esters. It is these capabilities and characteristics, that set lipases as significant players in a myriad of scientific applications.

Biosynth offers a large collection of lipases of microbial origin, available to purchase as unique individual lipase enzymes, or as part of our screening kits.

• Immobilized Lipase Kit - 7 unique lipase enzymes that are immobilized on a hydrophobic carrier and are suitable for reactions in organic solvents or solvent-free systems.
• Lipase Kit - 25 unique recombinant lipases with different pH and temperature optima and substrate specificity properties.

Lipase Suicide Inhibitors

To complement our lipase portfolio, Biosynth has a range of suicide inhibitor substrates for lipases. Specially designed for active site titration experiments, these products can be used for the quantitative determination of active lipases and esterases in complex protein mixtures. They are also suitable for in-house applications such as for quality control and sensitive detection of hydrolytic enzymes like lipases and esterases. The custom synthesis of suicide inhibitors with varying chain lengths and/or leaving groups is also available.

• Methyl 4-nitrophenyl hexyl phosphonate - Chromogenic standard substrate (405 nm) for the determination of active sites in lipases.
• Butyl 4-nitrophenyl hexyl phosphonate - Chromogenic substrate (405 nm) for the determination of active sites in lipases preferring long chain leaving groups.
• Octyl 4-nitrophenyl hexyl phosphonate - Chromogenic substrate (405 nm) for the determination of active sites in lipases preferring hydrophobic leaving groups.
• Methyl 4-methylumbelliferyl hexyl phosphonate - Fluorogenic standard substrate (λex: 363 nm λem 465 nm) for the determination of active sites in lipases.

X-Shining? Thermostable Luciferase

Biosynth’s modified thermostable X-Shining? Luciferase is perfectly suitable for any luciferin-luciferase-based assay using D-luciferin (dLuc) or synthetic pro-luciferins (caged luciferins). Examples of its typical applications include ATP tests in hygiene monitoring, ATP tests in drug screenings, and bacteria identification in microbial assays. Our new luciferase has been optimized by genetic engineering for strongly increased thermostability and storage stability. X-Shining? Luciferase is supplied as an aqueous solution with glycerol, and it may be kept for months at room temperature without significant loss of function. In temperature stress tests, the enzyme survives temperatures of 0°C for over an hour, whereas a normal luciferase from firefly is inactivated after only a few minutes.

X-Shining? all-in-one ATP Assay

The one-step X-Shining? all-in-one ATP Assay provides in a single vial an optimized reaction solution for the specific bioluminescent detection of ATP levels. Bioluminescence-based assays are extremely sensitive; a standard luminometer can detect as little as 0.1 picomole of ATP.

The measurement of ATP levels is crucial to study the growth of cell cultures, cell viability, cell response, and biochemical processes, to monitor environmental sample activity levels, assess water quality, test for biological contamination, monitor a broad range of enzymatic activities, and assess antibiotic efficacy and resistance in populations.

Find out more on our X-Shining? Luciferase webpage.

LacBuster?

LacBuster? beta-lactamases

Beta-lactamase enzymes have been 'villainized' in the biological and medicinal world. Their involvement in the current global crisis of antibiotic resistance is incriminating and has tarnished the use of the most commonly prescribed antibiotic class, the beta-lactams. Beta-lactamases provide bacteria with antibiotic resistance through their ability to hydrolyze the β-lactam amide of anti-bacterial. However, these enzymes can also be used in the development of new bacterial drugs and environmental monitoring. Biosynth manufactures these high-quality and high-performing beta-lactamases, delivering them to you in the form of LacBuster?-branded optimized formulations.

LacBuster? is a broad-range beta-lactamase formulation recombinantly produced in E. coli with activity towards penicillins, 4th and 5th-generation cephalosporins, and carbapenems. LacBuster? has been optimized for sterility testing and environmental monitoring in the manufacture and dosage formulation of beta-lactam antibiotics. See our range of LacBuster? products in our catalog.

LacBuster? Agar Plates For Environmental Monitoring

A general-purpose growth medium supplemented with LacBuster? to effectively neutralize beta-lactam antibiotics. Our contact and settle plates are suitable for the cultivation of a wide variety of microorganisms for environmental monitoring within the pharmaceutical industry as well as for high-performance monitoring of your isolators and clean rooms during beta-lactam manufacture.

Summary of Biosynth's Other Cutting-Edge Enzymes for Research

Peroxidases

Developed for high activity, specificity, and selectivity Biosynth peroxidases carry out a range of reactions: epoxidation, N-demethylation, halogenations, alcohol/aldehyde oxidation, and N- or S-oxidation. See our peroxidase kit, containing 2 recombinant peroxidases with an optimum temperature of 20-40 °C and an optimum pH of 5 and 8.?

Nitrile Hydratases

Ecofriendly metalloenzymes for the conversion of nitrile substrates into useful amide products.

Alongside our individual Nitrile hydratase enzymes, Biosynth has the Nitrilhydratase Kit, which contains the ten recombinant Nitrile hydratase enzymes.

Amidases

Biosynth amidases belong to the family of hydrolases, acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides.

• Amidase - From Pseudomonas aeruginosa, recombinant
• Amidase 02 - From Rhodococcus sp., recombinant

Isoamylase

Biosynth isoamylase belongs to the family of carbohydrolases acting specifically on α-1,6-glucosidic branch linkages in carbohydrates like amylopectin or glycogen.

• Isoamylase - From Pseudomonas sp.

Proteinases/Peptidases

• Carboxypeptidase Y - From S. cerevisiae, recombinant

Natural Tubulysins and Analogues

For use in conjugate therapeutics, our partner Tube Pharmaceuticals has developed several tubulysins produced at Biosynth by fermentation. These tubulysin toxins as a conjugate payload allow resistant cancers to be targeted. At Biosynth we have Tubulysin A and Tubulysin B available on a mg to g scale and other natural or synthetic analogues on request.

Other Bulk Enzymes for Pharma and Diagnostic Applications

• Trypsin Standard
• Sphingomyelinase
• Proteinase K
• Ultra Nuclease
• Endoglycosidase H
• T7 RNA Polymerase

Talk to our team today.

Biosynth are at the forefront of innovation and not only do we offer an exciting range of novel research enzymes but we also provide custom enzymes and state-of-the-art enzyme technologies namely our CTAT? enzyme for site-specific conjugation. Visit our enzyme webpage to discover Biosynth's high-performance enzyme solutions for custom applications in biopharma, biotech, and applied markets.