Next Generation 2-Photon Microscopy

2-photon Fluorescence Microscopy is Key in Bio Imaging

Since demonstrated by Winfried Denk and J Rudi Strickler , two-photon fluorescence microscopy has become a key technology in biological imaging. By offering reduced photobleaching, lower phototoxicity, increased imaging depth, and significant higher resolution in thick tissues as compared to standard fluorescence microscopy, two-photon microscopy found its way into a large array of applications, ranging from skin-tissues diagnostics to in-vivo imaging of individual neurons in the brain.?

Advantages of using single-wavelength fiber lasers

The unnecessity of wavelength tuning in two-photon microscopy and therefore the possibility to use fiber lasers strongly reduces the complexity in two-photon microscopy.?Over the last years, there has been a rapid development of mode-locked femtosecond fiber lasers emitting at a single-wavelength with the clear goal to provide a cost-efficient, simple, and easy-to-use alternative for two-photon microscopy applications.?

The unique offering of single wavelength fiber lasers have the potential to revolutionizing two-photon fluorescence microscopy by making simple and cost-efficient light sources available to everyone.

Simplifying Two-photon Microscopy

In contrast to conventional linear fluorescence microscopy, the nonlinear character and the longer excitation wavelength in multi-photon microscopy offer several advantages: larger probing depth lower phototoxicity and reduced photodamage.

Both advantages make 2p-fluorescence microscopy an ideal choice for the direct spatiotemporal visualization of neurons and neuronal activity in living animals. The key fluorescent proteins for such experiments are green and red fluorescent proteins that can be excited by 2-photon absorption at 920nm and 1050nm, respectively. The clear requirements in terms of wavelength, pulse duration, and output power make TOPTICA's easy-to-use and fully integrated fiber lasers?FemtoFiber ultra 920?at 920nm and?FemtoFiber ultra 1050?at 1050nm the perfect choice for 2-photon experiments in neuroscience.

"For me the?FemtoFiber ultra 920?with its Clean-Pulse Technology has the perfect optical parameters for highest two-photon image quality. In addition, the passive and cold laser head is an essential feature since it reduces the noise in laboratory and with that the stress on the living animals"

Dr. Weijian Zong , Moser Group, Kavli Institute for Systems Neuroscience

To understand the potential and latest trends in 2-photon fluorescence microscopy in neuroscience please watch the webinar with?Dr. Weijian Zong?from the?Moser lab?in Trondheim, pioneering the field of miniaturized 2-photon microscopy.

FemtoFiber ultra 920 – High Power Ultrafast Fiber Laser for 2-Photon Microscopy

The unnecessity of wavelength tuning in two-photon microscopy and therefore the possibility to use fiber lasers strongly reduces the complexity in two-photon microscopy. In addition, modern single-wavelength fiber lasers, come in an extremely compact, air-cooled package featuring several options that support the user in the experiment.

With more than sufficient output power, shortest pulses, and the Clean-Pulse Technology, the FemtoFiber ultra 920 features the highest relative peak power and enables unmatched brightness in 2-photon microscopy without unwanted heating of the sample.