NAR Breakthrough: A comprehensive analysis framework for evaluating commercial single-cell RNA sequencing technologies
NAR Nucleic Acids Research
Published by Oxford University Press - Impact Factor 16.7
by Marco De?Simone, Jonathan Hoover, Julia Lau, Hayley?M Bennett, Bing Wu, Cynthia Chen, Hari Menon, Amelia Au-Yeung, Sean Lear, Samir Vaidya, Minyi Shi, Jessica?M Lund, Ana Xavier-Magalh?es, Yuxin Liang, Ahmet Kurdoglu, William?E O’Gorman, Zora Modrusan, Daniel Le and Spyros Darmanis
Single-cell RNA sequencing (scRNA-seq) technologies help researchers understand gene activity in individual cells. Recent advancements offer various options, complicating the choice of technology. A systematic comparison of nine commercial scRNA-seq kits was conducted using a common reference sample from a single donor for consistency. The analysis evaluated cost, protocol duration and performance metrics at the library, gene and cell levels. This framework provides a basis for future assessments of emerging methods across diverse sample types. With data from over 169?000 cells, this study aids researchers in selecting a suitable scRNA-seq technology, paving the way for advancements in single-cell biology.