Mycoplasma simple detection method

Extract the RNA of the cells to be tested by Trizol method (recommended to use TRIpure Total RNA Extraction Reagent Cat. No.: EP013), reverse transcribed into cDNA?

Detection primer PCR detection of Mycoplasma contamination of cells.?

Mycoplasma detection primers

Mycoplasma_F01:

GGG AGC AAA CAG GAT TAG TAT CCC T

Mycoplasma_R01:

TGC ACC ATC TGT CAC TCT GTT AAC CTC

40 cycles, annealed at 63 degrees

Product size: about 200bp

Positive: Mycoplasma infection; Negative: Not infected

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ELK Biotechnology? has been deeply involved in the field of immunology for more than 10 years, and has accumulated a lot of experience in ELISA R&D and production. The existing technology and antibody pair reserves can produce more than 8,000 high-quality Elisa kits, covering more than 50 countries and regions around the world. It has won unanimous praise from the majority of scientific research users.

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Excellent product performance

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① High accuracy:The inter-assay difference and intra-assay coefficient of variation are both less than 9.9%

② High sensitivity: PG?level

③ High specificity: The cross-reaction rate is less than 5%

④ Excellent recovery rate: 85%--115%

⑤ High stability: After the reagents are stored at different temperatures, the maximum use time is up to 1 year