Maximize Biomanufacturing Yield and Efficiency with the Power of arProtein A Focurose HR Resin, Unleashing Purification Potential
Affinity resins were established and developed based on the principle of specific adsorption between biomolecules and other ligand molecules to achieve the purification of target molecules. Protein A is a staphylococcus cell wall protein with a molecular weight of 42 KD, which contains five Fc segments that bind specifically to the Fc segment of antibody lgG molecules. lt has five structural domains that bind specifically to the Fc segment of the antibody lgG molecule.
VDO has achieved a breakthrough with the development of arProtein A Focurose HR, a cutting-edge resin that combines VDO's modified Protein A ligand with cross-linked agarose. This innovative resin is specifically designed for the efficient separation and purification of ascites, cell culture supernatants, serum-derived monoclonal antibodies, multiple antibodies, and Fc-tagged proteins. Its remarkable features include high loading capacity, stable alkaline resistance, excellent purification capabilities, reliable reproducibility, and seamless substitution, which significantly reduce customer research and development costs while streamlining production processes.
Resin Properties
1.??????High rigidity Agarose base holder
2.??????Particle size uniformity ~ 75um
3.??????High alkali resistance 0.5M NaOH
4.??????Stable in all commonly used buffers
5. Anti-pressure low?
Technical Parameter
Note:
1.??????The binding load of the resin will vary depending on the source and isotype of the sample.
2.??????Resins that are immersed in the eluent for a long time will lead to hydrolysis of the ligand and eventually affect the resin loading.
Performance Characteristics?
When the retention time is ≥5 minutes, the loading capacity shows a slow-increasing trend. To ensure experimental time and loading capacity, it is recommended to set the retention time as 5 minutes. At this retention time, the packing material loading is above 60 g/L.
1. Static Alkali Resistance Test
Soaking in 0.1M NaOH for 1440 hours does not affect the DBC loading.
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Soaking in 0.5M NaOH and 1M NaOH for 168 hours results in a decrease in DBC loading of less than 20%.
1. Dynamic Alkali Resistance Test
After 200 cycles in 0.1M NaOH, the DBC remains essentially unchanged. After 100 cycles in 0.5M NaOH, the DBC maintains 80% of its initial value.
Using a 1.6cm diameter chromatography column, under the conditions of a column height of 26cm and a flow rate of 500cm/h, the pressure remains around 0.25Mpa, significantly below the maximum pressure tolerance of the packing material at 0.50Mpa.
Under the same column height and linear flow rate, our research-grade column exhibits comparable backpressure performance to the production-grade column.
VDOBIOTECH Company Profile
VDO Biotech Co., Ltd.?is an esteemed high-tech enterprise comprising a workforce of over 500 employees, dedicated to innovative microsphere technologies and the production of a variety of premium microsphere products catering to discerning customers worldwide. VDO is headquartered in the BioBAY of Suzhou Industrial Park in, China, with manufacturing facilities certified to ISO9001:2015 and covering more than 10,000 square meters. For any microsphere-related applications, our reliable team is the ideal partner.
Contact us:
Telephone：+86-15850246572
Email:[email protected]
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