March 2024 Edition
Check out the latest in proteomics research, including the latest publications featuring IonOpticks' famed Aurora Series products.
IMBAS-MS Discovers Organ-Specific HLA Peptide Patterns in Plasma
Immunopeptidomics, the study of peptides presented by human leukocyte antigen (HLA) proteins, has immense potential for biomarker discovery and understanding immune responses. However, current techniques are limited by complex workflows and low sensitivity, typically requiring large volumes of tissue or biological fluids. In this paper,? Maria Wahle et. al, introduce IMBAS-MS (Immunopeptidomics by Biotinylated Antibodies and Streptavidin), a highly sensitive, automated, and economical workflow for HLA peptide analysis from just 200 μl of plasma.
The key innovation of IMBAS-MS is the replacement of the traditional ProteinA/G-based antibody capture with a streptavidin-biotin system, simplifying the protocol and enabling generic use of biotinylated antibodies, in addition to multiplexing sample analysis using chemical labelling. Coupled with recent advancements in mass spectrometry, such as the Bruker timsTOF Ultra and data-independent acquisition (DIA) using predicted spectral libraries, IMBAS-MS quantifies over 5,000 HLA class I peptides in just 30 minutes, a substantial improvement over previous methods.
Through deep profiling of healthy donors, the authors demonstrate that the plasma immunopeptidome is remarkably stable over a year, exhibiting high reproducibility between individuals with overlapping HLA types. Intriguingly, immunopeptides originating from diverse tissues, except the brain, are proportionately represented, suggesting that the plasma immunopeptidome reflects system-wide changes in the human proteome.
This groundbreaking workflow paves the way for novel discoveries in immunology and precision oncology. IMBAS-MS could be used as a powerful tool for biomarker discovery, enabling minimally invasive monitoring of immune responses in various pathological conditions, from autoimmune disorders to cancer. With its enhanced sensitivity, throughput, and cost-effectiveness, IMBAS-MS could revolutionize the field of immunopeptidomics and become an invaluable asset in the pursuit of personalized medicine.
Dr Jarrod Sandow
Head of Product Development, IonOpticks
Leduc et. al have developed nPOP, a revolutionary method for single-cell proteomics that enables researchers to prepare thousands of high-quality single-cell samples with remarkable flexibility.Utilizing nanoliter volume droplets on glass slides, nPOP supports various multiplexed mass spectrometry methods. Implemented on the CellenONE instrument with readily available consumables ...?
In a groundbreaking study, researchers used Single Cell ProtEomics to delve into the intricate world of single-cell proteomics during epithelial-mesenchymal transition (EMT).?
This vital process influences various aspects of cell behavior and plays a crucial role in development, tissue repair, and disease progression. Unlike previous studies that focused on changes...
Presenting our latest application note, “Utilization of the speed, sensitivity and accuracy of the ZenoTOF 7600 system to enhance protein identifications from packed emitter columns.”
We thank Tony Purcell and his team at the Biomedicine Discovery Institute, Monash University for generating this data, which demonstrates the capabilities of the SCIEX ZenoTOF 7600 system when optimised with Aurora SX columns.
We’re excited to share more collaborations like these with leading experts in the future, showcasing the contributions of researchers and the role our technology plays in transforming research methods.
The IonOpticks Way: Meet Greta.
Transitioning all the way from Lithuania to Australia, she brings with her profound expertise in chemistry and a rich background in quality control and analytical chemistry. Greta's expertise encompasses scientific and quality control laboratory work, where she has conducted experiments, analysed data, and ensured adherence to stringent quality standards.??
Also this month:
In March, we welcomed two outstanding new additions to the IonOpticks team - Dr. TJ, joining our Product Development team, and Max, joins our Operations as Team Administrator. Their diverse talents and fresh perspectives have already invigorated our workplace. More on Max and TJ below.
At the US HUPO conference in Portland, our team drew a crowd with our latest round of 'cheeky swag' - with t-shirt designs that were an undeniable hit with the "Sensitive and Reliable Protein Junkies", sparking admiring glances and conversations everywhere we went. Events like this remind us why we do what we do - they're full of ever-evolving ideas that keep us at the leading edge, and provide us with prime opportunities to showcase our latest products.?
领英推荐
Following on from February’s company retreat,?the team?gathered for our first bi-monthly showcase for 2024 this month - where transparency and collaboration take centre stage. It’s the perfect opportunity for different areas of our business to stay connected and up to date with the remarkable work being done by individuals, teams, and the company as a whole. But that’s not all – we also had the pleasure of getting to know our newest recruits better as they present a little more about themselves! We’re always curious hearing their stories and learning about their unique backgrounds - both professionally and personally.
Welcoming fresh perspectives and talents into our team is what keeps us growing and innovating - and we’re bigger and better than ever before.We’ve been doing these showcases for a while now - yet each and every time we’re reminded of where we’ve come from, and importantly, where we’re going.?
What an exciting year we’ve got ahead of us: big product launches, expansion, and a travel calendar that would make any nomad’s eyes water!
Max Lassally , Team Administrator.
Originally from New Zealand, Max's journey has taken him across the globe before settling in Melbourne, for a second time.?
Max's background in sales and service spans diverse industries, including coffee, travel, health insurance, and medical equipment, offering up a world of experience to IonOpticks' sales functions.?Curious about new things and the 'why' and 'how', he uses his attention to detail to seek out opportunities for refinement, continuously improving the way he works.
Outside of work, Max enjoys cooking, which subsequently leads to indulging in delicious meals in his spare time. Eager to embark on a journey of continuous learning and professional development in a business that values innovation, Max looks forward to contributing his expertise to?#teamIO
Dr Todd (TJ) Blackburn , Product Manager.
A California native with a background in biochemical engineering and a PhD in neuroscience, specialising in drug development for rare epilepsy disorders.
With a passion for making a meaningful difference in the lives of others, he thrives on projects that directly impact consumers and patients.When not immersed in work, TJ can be found outdoors, training for triathlons and pushing his limits - with ambitious goals set for this year, including his first half ironman and second full marathon.
TJ is excited about leveraging his diverse skillset and background to contribute to IonOpticks' product pipeline. The constant influx of new challenges and objectives is incredibly motivating to him, and we believe he will be a perfect fit for our rapidly expanding R&D team.
Significant discounts off RRP,?with IonOpticks bulk column packs.
As you begin to allocate your provisional spending for the year, consider the smart, economical choice of Aurora Series bulk column packs.?
These packs are an investment in class-leading chromatography, and with significant discounts off RRP, you can fully equip your lab with the best consumables, and focus on delivering the best research possible.
Have you registered for the 25th International Mass Spectrometry Conference in Melbourne yet?
For those considering registration, remember that the early bird offer is available until May 17th, providing a chance to secure your attendance at a reduced rate.?
We encourage you to finalise your submissions and registrations soon to ensure your participation in what is shaping up to be an excellent conference, led by some of the best minds in the field.?
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