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Magnetic Bead: What is it?

Genfine Biotech (Changzhou) Co., Ltd

Our Genes Make Life Fine.

发布日期: 2022年6月29日

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Overview

Magnetic beads consist of tiny iron oxide particles (20 to 30 nm), such as magnetite (Fe3O4), which are superparamagnetic. Superparamagnetic beads differ from common ferromagnets beacuse they only appear magnetic properties in the presence of an external magnetic field. This property depends on the size of the particles in the bead and enables the bead and any material bound to it to separate in suspension.?Since they don't attract each other outside of a magnetic field, they can be used without worrying about unwanted clumping.
There are many types of magnetic beads available.?Different coatings and chemistries give each type of bead its binding properties that can be used for the magnetic separation of nucleic acids, proteins, or other biomolecules in an easy, efficient, and scalable manner.
The ease of use makes it easy to automate and is ideal for a range of applications including sample preparation for next-generation sequencing (NGS) and PCR, protein purification, molecular and immunodiagnostics, and even magnetic activated cell sorting (MACS) Wait.

What is magnetic separation?

Magnetic separation uses a magnetic field to separate micron-sized paramagnetic particles from a suspension. In molecular biology, magnetic beads provide a simple and reliable method to purify various types of biomolecules, including genomic DNA, plasmids, mitochondrial DNA, RNA, and proteins. The main advantage of using magnetic beads is that you can isolate nucleic acids and other biomolecules directly from crude samples as well as from a variety of different types of samples.

How does magnetic bead?DNA extraction work?

Magnetic beads have been around for decades. Their potential in nucleic acid purification was recognized as demonstrated by the 1990 US patent.After binding the DNA, an external magnetic field attracts the magnetic beads to the outer edge of the tube, immobilizing them. When the beads are immobilized, the bead-bound DNA is retained during the washing steps. Elution buffer is added, and the magnetic field is removed, releasing the DNA as a purified sample ready for quantification and analysis.

This method eliminates the need for vacuum or centrifugation, minimizes shear forces on target molecules, requires fewer steps and reagents than other DNA extraction protocols, and is suitable for use in 24, 96, and 384 well plates automation.