Last Chance for Pittcon HPLC short course registration for Thursday. Free textbook!

All-inclusive HPLC short course starting 3/4/21 from Pittcon. Free textbook offer!

Pittcon and I will offer a FREE HPLC Textbook for ALL HPLC Pittcon short course registrants. The is a 2-day all-inclusive HPLC course for practicing scientists (online, live, intermediate level) on March 4 and March 8, 2021. The cost will be $800 for a 1-day session ($400 for students, with an option to attend one or both days). Day 1 is on fundamentals, and Day 2 on practices (applications, method development, and troubleshooting). The focus is on pharmaceutical and biopharma analysis, though other applications are covered.

Here are the registration link, course description, and FREE textbook offer.

Registration link: Pittcon 2021 Short Course: Session: HPLC and UHPLC for Practicing Scientists 1: Fundamentals (PART 1 of 2) (a2zinc.net)

A FREE Textbook:

M. W. Dong, HPLC and UHPLC for Practicing Scientists, 2nd Ed., Wiley, NJ, 2019.

HPLC and UHPLC for Practicing Scientists: 9781119313762: Medicine & Health Science Books @ Amazon.com

A choice of an E-book (for PC, reader, or tablets) or paperback, a $99.95 value.

Note the first edition published in 2006 is a bestseller HPLC book with 9000 copies sold. The second edition was published in July of 2019 and has 70% more content and three new chapters on biopharmaceutical quality control, LC/MS, and UHPLC.

HPLC and UHPLC for Practicing Scientists 1 and 2:

Fundamentals, Method Development, and Troubleshooting

Dr. Michael W. Dong, Norwalk CT, USA (2-day or four 4-h sessions),

[email protected]

Course description

This intermediate, all-inclusive 2-day (or six 2.5-h webinars) workshop will provide the analytical scientist with a clearer understanding and a solid working knowledge of the concepts, instrumentation, columns, applications, and best practices in method development and troubleshooting of HPLC and UHPLC (ultra-high-pressure liquid chromatography). The focus is on pharmaceutical analysis of small molecule drugs. 

Who Should Attend?

Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want to get an updated overview of the fundamentals of HPLC and UHPLC in pharmaceutical analysis and other applications. A basic understanding of chemistry and HPLC with some hands-on experience is assumed.

Day 1: HPLC 1: Fundamentals

1. Concepts 

A.   Introduction: The chromatographic process, advantages and limitations, and chromatographic modes (NPC, RPC, IEC, SEC)

B.   Concepts: Retention factor (k), separation factor (a), column efficiency (N), resolution (Rs), tailing factor (Tf), the resolution equation, the linear solvent strength theory, and column void volume (VM) 

C.  Mobile phase factors (organic modifiers, pH, buffers), mass spec compatibility, isocratic and gradient separations, operating parameters (Flow, gradient time (tG), column temperature (T)), peak capacity (Pc) 

2.   HPLC Columns, Trends, and Selection Guides  

A.   Column fundamentals: Glossary, Characteristics, and types, packing characteristics (support type, particle size, pore size), bonded phases, end-fittings, plate height (H), the van Deemter equation

B.   Trends: Trends of shorter and smaller columns and the use of high-purity silica, traditional and novel bonding chemistries, the concept of “orthogonality,” listing of common RPC columns, column selection guide 

C.  Newer column types: Hybrids, charged surface hybrids, HILIC, sub-3 and sub-2 mm, and superficially porous particles (SPP)

1. HPLC instrumentation and Operating Principles  

A.   The system, Pump, and Autosampler. Integrated vs. modular system, solvent delivery system, low-pressure vs. high-pressure mixing, dwell volume, pump trends, autosampler types, and trends 

B.   Detectors: Operating principles of UV/Vis detection and characteristics,  photodiode array detector (DAD), newer detectors for non-chromophoric analytes (ELSD, CAD, and CLND)

C.  MS, CDS, and Instrument Bandwidth. The mass spectrometer (MS), types and ESI interface, Data Chromatography System (CDS), peak integration strategy, instrumental bandwidth

Day 2: HPLC 2: Applications, UHPLC, Method Development, and Troubleshooting

4.   HPLC Applications and an Overview of UHPLC

1. HPLC applications including pharmaceutical, biopharmaceuticals, food, environmental, chemical, and bioseparations.
2. UHPLC overview, perspectives, and benefits: UHPLC concepts, instrumentation, and benefits (fast separations, high-resolution analysis, and rapid method development)
3. Potential issues and how to mitigate (viscous heating, operating nuances, compatibility to existing methods, injection precision, detector sensitivity vs. mixing volumes), method translation from HPLC to UHPLC, the transition from HPLC to UHPLC. 

5.   HPLC Method Development and Validation          

A.   Tradition strategy for HPLC method development and a survey of automated tools and software (DryLab, ACD AutoChrom, and Fusion QbD)

B.   3-pronged template approach for rapid method development

C.  Use of a universal generic gradient method for the assay of multiple NCEs and stability-indicating assays with simple adjustments

D.  A brief overview of method validation and method transfer with case studies.

6.   HPLC Operation and Troubleshooting

A.   Mobile phase and sample preparation and best practice in HPLC operation

 B.   Maintenance and troubleshooting guide with case studies

C.  Diagnosing and solving problems (pressure, baseline, peak, data performance)

Biography

Dr. Michael W. Dong is a principal consultant in MWD Consulting, focusing on consulting and training services on HPLC, pharmaceutical analysis, and drug quality. He was formerly Senior Scientist in Analytical Chemistry and Quality Control at Genentech, Research Director at Synomics Pharma, Research Fellow at Purdue Pharma, Senior Staff Scientist at Applied Biosystems/Perkin-Elmer, section head at Celanese Research Company, and postdoctoral research fellow at Naylor-Dana Institute for Disease Prevention.

He holds a Ph.D. in Analytical Chemistry from the City University of New York and a certificate in Biotechnology from the University of California Santa Cruz. He has 130+ publications and five books, including a bestselling book on chromatography (HPLC and UHPLC for Practicing