Lab Notes Newsletter

Welcome to the December edition of our newsletter!

For 40 years, we’ve helped scientists harness the full potential of biology with next-generation technology. Here we share the latest in automated, end-to-end solutions that span research disciplines to advance scientific discovery and improve the quality of human life worldwide.

eBOOK - CRISPR Cas9 Gene Editing: The Art of DNA Design

Explores one of the most revolutionary advancements in genetics: CRISPR-Cas9 gene editing. Clustered Regularly Interspaced Short Palindromic Repeats, known simply as CRISPR, this technology marks not just a scientific milestone but a fundamental shift in biological research.

Whether you are a researcher, medical professional, policy maker, academic, or simply a curious reader, this introduction will provide a deeper understanding of how CRISPR-Cas9 could fundamentally transform our approach to gene editing for the better.

This eBook, CRISPR Cas9 Gene Editing: The Art of DNA Design, offers a deep dive into the science, applications, and future possibilities of this revolutionary technology. Whether you're focused on healthcare, agriculture, or industrial applications, this 28-page guide?provides the insights you need to stay ahead in the rapidly evolving field of synthetic biology.?

Download eBook

ON-DEMAND WEBINAR?- Endotoxin testing: ensuring safety, compliance, and precision with microplate readers

In case you missed it, or would just like to re-watch the event, our recent webinar,?Adapting your laboratory's microplate reader for bacterial endotoxin testing?is?now available to view on-demand.

In this webinar,?we discuss the Bacterial Endotoxin Test (USP 85), the standard test for ensuring safety from bacterial endotoxins in pharmaceuticals and medical devices.

Key takeaways include:

• Understanding the Bacterial Endotoxin Test (USP 85):?Gain insights into the purpose and importance of the Bacterial Endotoxin Test in ensuring the safety of pharmaceuticals and medical devices, and how it is implemented using the Limulus amebocyte lysate (LAL) reagent
• Importance of Kinetic Analysis:?Learn why kinetic analysis is preferred for its low sensitivity and broad quantitative range, and understand the equipment requirements for this analysis, including absorbance readers with incubation and kinetic capabilities
• Regulatory Compliance and Data Integrity:?Discover essential FDA and regulatory requirements for data compliance and integrity in endotoxin testing, covering the importance of secure electronic records, approvals, and software reporting

Watch the webinar

APPLICATION NOTE?- AI-enabled hit selection of drug screening on human pancreatic cancer organoids

Pancreatic ductal carcinoma (PDAC) is a highly aggressive cancer and the 4th leading cause of cancer mortality in the United States.

Despite advancements in understanding PDAC, effective therapies remain elusive. Patient-derived organoids (PDOs) offer promise for ex vivo testing since they retain tumor histopathology and gene mutations. However, manual cell culture, lengthy imaging protocols, and limited automation hinder their use in screening.

To overcome these challenges, we established an automated, machine learning-enabled compound screening workflow with PDOs using the Gri3D? platform from SUN bioscience. The data revealed significant cytotoxic effects of certain compounds and demonstrated the potential for performing drug screening using robust and unbiased data analysis.

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APPLICATION NOTE - A microwell platform to standardize human rectal organoid cultures for high-content imaging and phenotypic analyses

Enhance the flexibility and robustness of your image analysis pipeline with AI-based segmentation

We’ve come a long way since the development of the first mouse intestinal organoids in 2009. However, organoid models still heavily rely on the use of solid extracellular matrix (ECM). This conventional culture method introduces a high level of heterogeneity in terms of size, shape, and distribution of organoids which complicates subsequent downstream readouts and image analyses.

In this application note, we share how you can overcome these challenges—and closely follow human rectal organoid development—using ready-to-use Gri3D? technology. This platform enables the homogeneous generation of a single microtissue in each microcavity in suspension-like conditions, without the need of a solid ECM.

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TUTORIAL SERIES - Transform your Patch-Clamp Research: Uncover essential skills and techniques with our expert-led Axon Patch-Clamp electrophysiology tutorial series

Whether you’re just starting out or looking to refine your expertise, this series provides a thorough exploration of electrophysiological techniques, measurements, and the complete setup for recording and analysis. Experienced researchers will benefit from advanced discussions on specialized methods and noise management, ensuring you master every aspect of the technique.

Our team of experts will guide you through practical examples and real-life applications, demonstrating how the Axon Instruments? portfolio serves as a complete workflow solution for patch-clamp electrophysiology.

Explore the tutorial series

EVENTS

Cell Bio (ASCB)?

December 14-18, 2024 | San Diego, CA