Knockouts vs Knockins. The use of target gene editing in preclinical research
In our latest blog, we explore how CRISPR/Cas9 technology has revolutionized genetic engineering with Knockouts and Knockins. These techniques enable precise deletion and insertion of DNA sequences, unlocking new possibilities in preclinical research. Knockouts eliminate DNA sequences, while Knockins insert new sequences, all thanks to the functions of CRISPR enzymes. These tools are crucial for understanding genetic functions and modeling diseases in animal models like zebrafish.
In this post, we go over the various knockout and knockin techniques available and their distinctions, helping you select the most suitable one for your project. We'll also explore the primary applications of each method, enabling you to make the best choice based on your scientific objectives.
Read the full blog HERE.
New scientific publication introducing “A Zebrafish-Based Platform for High-Throughput Epilepsy Modeling and Drug Screening in F0"?
Last week, our recent work was accepted for publication in the International Journal of Molecular Sciences (@MDPI).
We present an innovative and optimized strategy for rapid and high-throughput generation of knockout zebrafish models for target identification/validation in epilepsy and screening of antiepileptic drugs (AEDs).
By co-inactivation of the target gene and an endogenous reporter locus we developed a method to efficiently select mutant animals.
In just three months, we can screen your candidate genes and molecule library to identify targets and evaluate compound efficacy. The time-saving and high-performance advantage offered by this platform opens up multiple applications, including personalized medicine.
Access the full paper HERE.
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Featured Research Solutions:
ZeClinics' zebrafish Crispants are a fast-track choice for screening loss-of-function alleles, and validating functional genetic models before committing to building a stable line. The Crispant approach provides early proof-of-concept or viability testing of your gene knockout model in as little as one month. Our zebrafish Crispants will enable you to reduce costs, time and animal use in your gene knockout projects.
Behavioral Phenotyping
High-throughput testing of zebrafish larvae behavior in multi-well plates. Sensory, motor, and cognitive functions can be tested in response to specific stimuli and at baseline.
Morphological and Functional Phenotyping
Robotic technologies for whole zebrafish organ-level imaging that allows the evaluation of single individuals in a high throughput manner. We can evaluate multiple morphological phenotypes, cardiovascular parameters, hepatic changes, etc. High-throughput screening is coupled with automated phenotyping extraction for more accurate and unbiased results.
We want to hear from you!
Contact our team of genetic experts to learn more about how we can assist you in your next upcoming project today.
Animal Welfare, OneHealth, OneWelfare and Vetrinary Advisor,
11 个月That′s very good news and work!