Jeju Techno Park, research results on Jeju’s native plant Damnacanthus major callus
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Jeju Techno Park (Director Moon Yong-seok, Jeju TP) Jeju TP Biodiversity Research Institute won Excellence Award for its metabolite analysis poster research on ‘Changes in the production of indicator substance rubiadin according to callus culture conditions’ during the 2024 Korean Metabolomics Society regular conference held at EXCO in Daegu, Korea.
Damnacanthus major Siebold & Zucc is a plant native to Jeju that belongs to the genus Hoja in the family Apophyllidae. Its leaves are egg-shaped and larger than those of the same genus, and the thorns are about 1 cm long. The root part of the tree is also used for medicinal purposes.
The Biodiversity Research Institute presented this research results using Damnacanthus major callus (Jeju native plant) as part of the Regional Cooperation Innovation Growth Project sponsored by the Ministry of Trade, Industry and Energy.
This study includes the establishment of a callus (wound tissue) cell line culture process following methyl jasmonate treatment, confirmation of indicator component content, and analysis of primary and secondary metabolites. They succeeded in establishing an indicator component by confirming rubiadin, a component that had not been detected in plant extracts, in callus of the tree.
In addition, it was confirmed that when methyl jasmonate was treated with fertilized callus, the content of not only rubiadin but also the phenolic components quinic acid and kaempferol increased, suggesting that this was due to abiotic stress. The results of changes in the composition of neck callus were also revealed.
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This study is meaningful in that it laid the foundation for the development of the plant cell line industry by stably producing fertilized callus through tissue culture and analyzing the production mechanism of indicator components which can be useful components for use as industrial raw materials. The value of use as cosmetic material will be further increased.
The Biodiversity Research Institute published a paper on the results of this research early this year in ‘Plants’, an internationally renowned academic journal in the field of botany published by MDPI, a large-scale Swiss academic journal publishing company.
Yong-hwan Jeong, head of the Jeju TP Clean Bio Business Division, said, “We have once again set a turning point in promoting the Jeju-style bio industry through analysis of the operating principles for producing useful ingredients from Jeju native plants.” He added, “Based on the results of this research, we will utilize the Jeju Cell Line Bank. We will strengthen research and development of bio-industrial materials and further grow Jeju bio industry by securing original technology.”
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