Isolation of genomic DNA from fungi.
VIDYANAND JHA
“Hi, I am VIDYANAND JHA, ‘??????????-??????????????????’ Master ???????? SRK” I’m working on my linkedin bio. Check again after ten years.
Introduction
Fungal genomes are among the smallest genomes of eukaryotes. The sizes of fungal genomes range from less than 10 Mbp to hundreds of Mbp. The average genome size is approximately 37 Mbp in Ascomycota, 47 Mbp in Basidiomycota and 75 Mbp in Oomycota.
The sizes and gene numbers of the smallest genomes of free-living fungi such as those of Wallemia ichthyophaga, Wallemia mellicola or Malassezia restricta are comparable to bacterial genomes. Some fungi exist as stable haploid, diploid, or polyploid cells, others change ploidy in response to environmental conditions and aneuploidy is also observed in novel environments or during periods of stress
Principle
The method involves disruption of fungal cells by employing homogenizer followed by inactivation of proteins using CTAB/Proteinase K. Further purify by chloroform: