HPLC and UHPLC for Practicing Scientists 1 and 2: Fundamentals, Method Development, and Troubleshooting

HPLC and UHPLC for Practicing Scientists 1 and 2: Fundamentals, Method Development, and Troubleshooting

EAS Two-Day in-person Course (You can register for either HPLC1 or HPLC2, though a two-day course is the best deal).?

Sunday, Nov. 13 & Monday, Nov. 14; 8:30am – 5:00pm

Dr. Michael W. Dong, MWD Consulting, Norwalk, CT

Registration link:?2022 Short Course Schedule - EAS 2022

Cost: $1175/2 day or $775 each day.?$70/day for full-time students.

The registration fee will include breakfast, two coffee breaks, and a buffet lunch each day. EAS short course provides one of the best values in live analytical chemistry training, particularly to those attending EAS Conference.

COURSE DESCRIPTION

This intermediate course will provide the analytical scientist with a clearer understanding and a solid working knowledge of the concepts, instrumentation, columns, applications, and best practices in method development and troubleshooting of HPLC and UHPLC (ultra-high-pressure liquid chromatography).?The focus is on the pharmaceutical analysis of small molecule drugs.

WHO SHOULD ATTEND

Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want an updated overview of fundamentals and best practices of modern HPLC/UHPLC in pharmaceutical analysis and other applications including HPLC method development, operation and operation, and troubleshooting.?A basic understanding of chemistry and HPLC with some hands-on experience is assumed.

Day 1: HPLC 1: Fundamentals:

1. Concepts

a. Introduction: The chromatographic process, advantages and limitations, and chromatographic modes (NPC, RPC, IEC, SEC)

b. Concepts: Retention factor (k), separation factor (a), column efficiency (N), resolution (Rs), tailing factor (Tf), the resolution equation, the linear solvent strength theory, and column void volume (VM)

c. Mobile phase factors (organic modifiers, pH, buffers), mass spec compatibility, isocratic and gradient separations, operating parameters (Flow, gradient time (tG), column temperature (T)), peak capacity (Pc)

2. HPLC Columns, Trends, and Selection Guides

a. Column fundamentals: Glossary, Characteristics, and types, packing characteristics (support type, particle size, pore size), bonded phases, end-fittings, plate height (H), the van Deemter equation

b. Trends: Trends of shorter and smaller columns and the use of high-purity silica, traditional and novel bonding chemistries, the concept of “orthogonality,”?listing of common RPC columns, column selection guide

c. Newer column types: Hybrids, charged surface hybrids, HILIC, sub-3 and sub-2 mm, and superficially porous particles (SPP)

3. HPLC Instrumentation and Operating Principles

a. System, Pump, and Autosampler. Integrated vs. modular system, solvent delivery system, low-pressure vs. high-pressure mixing, dwell volume, pump trends, autosampler types, and trends

b. Detectors: Operating principles of UV/Vis detection and characteristics, photodiode array detector (DAD), newer detectors for non-chromophoric analytes (ELSD, CAD, and CLND)

c. MS, CDS, and Instrument Bandwidth. The mass spectrometer (MS), types and ESI interface, Data Chromatography System (CDS), instrumental bandwidth

Day 2: HPLC 2:?Applications, UHPLC, Method Development, and Troubleshooting

4. HPLC Applications and an Overview of UHPLC

a. HPLC applications including pharmaceutical, biopharmaceuticals, food, environmental, chemical, and bioseparations

b. UHPLC overview, perspectives, and benefits: UHPLC concepts, instrumentation, and benefits (fast separations, high-resolution analysis, and rapid method development)

c. Potential issues and how to mitigate (viscous heating, operating nuances, compatibility to existing methods, injection precision, detector sensitivity vs. mixing volumes), method translation from HPLC to UHPLC, the transition from HPLC to UHPLC

5. HPLC Method Development and Validation

a. Tradition strategy for HPLC method development and a survey of automated tools and software

b. 3-pronged template approach for rapid method development

c. Use of a universal generic gradient method for the assay of multiple NCEs and stability-indicating assays with simple adjustments

d. A brief overview of method validation with case studies

6. HPLC Operation and Troubleshooting

a. Mobile phase and sample preparation and best practice in HPLC operation

b. Maintenance and troubleshooting guide with case studies

c. Diagnosing and solving problems (pressure, baseline, peak, data performance)

ABOUT THE INSTRUCTOR

Dr. Michael W. Dong is a principal of MWD Consulting, focusing on training and consulting on HPLC/UHPLC, method development, and pharmaceutical analysis.?He was formerly Senior Scientist at Genentech, Research Fellow at Purdue Pharma, and Senior Staff Scientist at Applied Biosystems/Perkin-Elmer.?He holds a Ph.D. in Analytical Chemistry from the City University of New York and a certificate in Biotechnology from the University of California of Santa Cruz. He has 130+ publications, including a bestselling book on chromatography (HPLC and UHPLC for Practicing Scientists, Wiley). He is an editorial advisory board member of LCGC North America, American Pharmaceutical Review, and the Chinese American Chromatography Association.

LinkedIn Connection

LinkedIn profile: https://www.dhirubhai.net/in/michael-dong-%E5%94%90%E7%BA%AC%E4%B8%AD-8390548/

MWD Consulting website: https://www.mwd-consulting.com/

Your connection to LinkedIn is welcome, and many HPLC and pharm analysis resources, including 30+ white papers, are available for free download on the website.

Recommended textbook:

M. W. Dong, HPLC and UHPLC for Practicing Scientists, 2nd Ed.,?Wiley, Hoboken, New Jersey, 2019. The list price is $103 though it can be purchased at Amazon.com at a substantial discount for either the paperback or the E-book. A limited copies of this bestseller book (paperback or eBook) will be available at a cost of $65 on-site. The short course will provide a quick overview of the fundamentals of the subject, while the textbook is a systematic and detailed reference source for concepts, best practices, and case studies, of HPLC focusing on pharma and biopharma applications.