How to Remove Image Blur in Thick Samples without Going Confocal
David Pe?a
Making the world safer and healthier through pioneering innovations on light microscopy. Seeing is Solving.
Widefield fluorescence microscopes offer high-resolution imaging for thin samples. But when it comes to thicker samples, widefield microscopes are limited in their imaging ability due to background fluorescence causing image blur.?
So, how do you remove image blur in thicker samples? The answer lies in optical sectioning. This method effectively overcomes the limitations of widefield imaging but has traditionally required a confocal system or deblurring software.?
Today, optical sectioning technology is now on research slide scanners. This means researchers can benefit from confocal capabilities for imaging thick samples—with significantly improved throughput. Here we explore the advantages of this optical sectioning method in detail while comparing it to traditional techniques.?
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