How to manually clean the chromatographic injection bottle

??Method 1

1. Pour out the test solution in the chromatographic sample bottle.

2. Immerse everything in 95% alcohol, wash with ultrasonic twice and then pour it dry, because alcohol easily enters the 1.5mL vial and is miscible with most organic solvents.

3. Pour in clean water and ultrasonically wash twice.

4. Pour out the lotion in the bottle and bake at 110 degrees Celsius for 1 to 2 hours. Never bake at high temperatures.

5. Cool and save.

??Method 2

1. Rinse with tap water several times.

2. Put it into a beaker filled with pure water and sonicate for 15 minutes.

3. Change the water and ultrasonic for another 15 minutes.

4. Soak in a beaker filled with absolute ethanol.

5. Finally, take it out and let it dry naturally.

??Method 3

1. Soak in methanol (chromatographically pure) and clean ultrasonically for 20 minutes, then drain the methanol.

2. Fill the chromatographic sample bottle with water, clean it ultrasonically for 20 minutes, and then drain the water.

3. Finally, dry the chromatography sample bottle.

??Method 4

1. Generally, it is rinsed and dried with clean water first and then soaked in potassium dichromate sulfate lotion.

2. The cleaning method of chromatographic sample bottles is the same as that of making liquid phase. First, soak it with medical alcohol for more than 4 hours, then ultrasonic for half an hour, then pour out the medical alcohol, use water for half an hour, and rinse with water. Then dry it.

??Method 5

1. If the cost is sufficient, it is best to use new ones every time.

2. If it is to be reused, the cleaning method is also very important. Soak in strong oxidizing cleaning solution (potassium dichromate) for 24 hours.

3. Clean it three times with deionized water under ultrasonic conditions, and finally clean it once with methanol, dry it and then use it.

4. The bottle pad must be replaced with a new one, especially when analyzing pesticide residues, otherwise it will affect the quantitative results.

??There are many problems with manual cleaning

1. The cleaning efficiency is low, the washing time is long, and it consumes time and energy. In addition, the brushes and detergents are used in large quantities and are difficult to manage, which wastes water resources.

2. Cleaning can easily leave dead ends, making it difficult to guarantee the cleaning effect, affecting test results and increasing manpower and material costs.

3. Manual cleaning has safety hazards and can easily cause environmental pollution. Some laboratories use ultrasonic cleaning machines. This cleaning method improves cleaning efficiency and cleanliness to a certain extent, but it still has unavoidable disadvantages.

The general cleaning process of an ultrasonic cleaning machine: first rinse with clean water, then soak in industrial ethanol, then clean twice with ultrasonic waves, then soak in clean water, and then clean twice with ultrasonic waves. Sometimes it is necessary to soak in strong acid, soak and clean repeatedly. Until washed. The cleaning process is cumbersome, requiring manual water changes many times and semi-automatic operation. At the same time, the ultrasonic cleaning machine is noisy and harsh when working, the cavitation effect is too strong and it is easy to break bottles and dishes, and the cleaning time is difficult to control.

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