HOPAX BIS-TRIS Buffer
Bis-Tris is one of the most popular zwitterionic biological buffers and it is often used in biological and biochemical research. Although Bis-Tris is not officially a Good’s buffer, its structure is very similar to?the buffers developed by Norman E. Good.
The origin of the name Bis-Tris comes from its structure. Unlike most of the other available biological buffers, Bis-Tris’ has functional groups related to?two different structural families: the Bis(2-hydroxyethyl)amine family and the Tris family.
We analyzed the scientific literature available to make a list of how Bis-Tris has been used and which concerns you should have before using it.
Bis-Tris Buffer basic information:
- CAS Number:?6976-37-0
- Molecular Weight: 209.2g/mol
- Formula: C8H19NO5
- Useful ph range: 5.8 – 7.2
- pKa (25°C): 6.46
What is Bis-Tris recommended for?
- Used as a buffer for anion exchange chromatography1
- Used as a running buffer, gel buffer, and sample buffer with different types of electrophoresis2,3
- Used with ACES as a running buffer for gel electrophoresis (Bis-Tris/ACES)4
- Used for agarose gel electrophoresis5
Which concerns should you have before choosing Bis-Tris for your research?
- It forms a strong complex with Pb2+ and Cu2+ and has a weak interaction with other common metals
- It is not suitable for use with the Bicinchoninic Acid (BCA) Assay.
- It interacts with human liver fatty acid binding protein (FAB) and affects protein dynamics6
领英推è
Useful tips about Bis-Tris:
- It is highly?soluble in water
- It is considered a good alternative to cacodylate, a highly toxic buffer
- It is considered a good alternative to maleic acid, a buffer with high UV-absorption
- It is considered a good alternative to citrate, a buffer that binds to some proteins and forms complexes with some metals
Further reading:The uses of Tris buffer - A starting guide
?
Hopax Bis-Tris Buffer
Hopax Fine Chemicals is among the largest producers of Bis-Tris in the world. Our products are shipped daily to top research centers and biotech companies in Europe, America, and Asia.
What we offer:
- Product straight from our manufacturing sites
- Worldwide shipping to your door
- Assistance with shipping
- Small and bulk packages (from grams to tons)
- International quality standards
- Standard and MBG Grade with Customized Specifications
- After-sales service with English-speaking staff
Visit our Website
For Bis-Tris - https://www.hopaxfc.com/en/product/bis-tris-buffer
For our other Biological Buffers - https://www.hopaxfc.com/en/product/category/biological-buffers