HIV "vif" gene's product investigation
Omid Nilchi zadeh rahbar
Microbiologists / Biotechnologist; Tech Development; Solution making...
Today I would like to share what I learned by https://www.cbs.dtu.dk to find protein's sequence using FASTA format resulted from NCBI GENEIDs...
This is human Immunodeficiency virus 1 Genome information
By clicking on RefSeq of NCBI, you will navigate to this screen...
Scroll down to proceed into "vif" geneID...
/gene="vif"
/locus_tag="HIV1gp3"
/db_xref="GeneID:155459"
Here??????????
Now click on GeneID, and proceed to next step...
Click on FASTA format to get this known-among applications format... It's good to have FASTA for sequences cause it's kind of transition format to keep forward...
After clicking on FASTA you will find this...
Copy the FASTA format sequence and open another browser tab for next step ??
Go to this precious link: https://www.cbs.dtu.dk
Choose the "CBS BIOINFORMATICS TOOLS" option... You will find the page below.... keep going forward and click on "VirtualRibosome" ...
On this page you can paste the "FASTA format sequence" which you copied before.... And then click on query bottom...
You will find protein's sequences as below...
This is "vif" protein's sequence and we can check the domains which is best for molecular targeting inside the cells (for example)
We can do the same procedures for gp120, gp41 and other viral parts of HIV which are more exposed on viral surfaces...
Have a Matrix time & chase your dreams...??
Omid