HDX-MS and Artificial Intelligence for Antibody Epitope Mapping

In June’s edition of De Novo Digest, we are focusing on HDX-MS, a powerful proteomics tool that employs mass spectrometry to identify antigen-antibody interactions with high resolution.

Upcoming Webinar: Integrating AI and HDX-MS for Antibody Epitope Mapping

Join us for our upcoming webinar, in partnership with MAbSilico , where we will demonstrate the application of AI and HDX-MS in screening and characterizing neutralizing antibodies against SARS-CoV-2 from an antibody discovery campaign involving healthy, vaccinated donors.?

• Computational modeling was used to screen over 700 mAbs from the discovery campaign, selecting 16 mAbs for expression.?
• This led to the identification of 12 binders, including 4 high-affinity binders and 1 cross-neutralizing antibody against several SARS-CoV-2 variants.?
• HDX-MS epitope mapping confirmed the binding epitopes, providing crucial structural details on antibody-antigen interactions and revealing further insights into antibody function.

???July 11, 2024

?10:00 AM EST / 16:00 CET

Register at the event below!

Antibody Characterization Service Giveaway!

We’re giving away one free antibody characterization services to our followers!

Why? We’ve launched new LC-MS services and we want the world to know:

?Rapid peptide mapping, enhanced peptide mapping, glycan analysis, disulfide bond analysis, PTM analysis, and sequence variant analysis.

What you win:

• One of the above LC-MS characterization services for an antibody. See promotion details.

How to win:

1??Follow Rapid Novor on LinkedIn

2??“Like” and “Share” this post below to your feed!

3??Stay tuned for the announcement on July 31?

Share this post for a chance to win?

See promotion details here

HDX-MS Epitope Mapping vs. Mutagenesis

Identifying antibody epitopes and paratopes is crucial for antibody development. Alanine scanning mutagenesis, which systematically substitutes individual amino acids to pinpoint essential residues for binding, is a common method for epitope mapping. Another method, hydrogen-deuterium exchange mass spectrometry (HDX-MS), measures the exchange rate of hydrogen with deuterium to analyze protein interactions.

Using mutagenesis epitope mapping and HDX-MS in parallel can validate epitopes and provide comprehensive information on the antibody and antigen. Each method has its own advantages and disadvantages, which should be carefully considered based on the antigen, antibody, development stage, and available resources.

Check out our recent article on comparing HDX-MS with alanine scanning mutagenesis for antibody epitope mapping.

Conferences and Events

We had a great time at #ASMS2024 last week! Did you catch our new poster??

We de novo sequenced functional and neutralizing antibodies directly from human plasma after SARS-CoV-2 vaccination using mass spectrometry.?

Catch us at these upcoming events. Will you be there? Let us know!

• HubXChange AbTx | June 17, Boston, MA
• Antibody Industrial Alliance 2024 | June 20 - 21, Montpellier, France
• ADLM 2024 | July 28 - August 1, Chicago, IL

Rapid Comics

Play the match-making epitope game with HDX-MS! ??

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