HDR vs NHEJ in CRISPR Gene Editing

HDR vs NHEJ in CRISPR Gene Editing

Understanding DNA Repair Pathways

Our latest article delves into DNA repair pathways, focusing on Homology Directed Repair (HDR) and Non-Homologous End Joining (NHEJ) in the context of CRISPR/Cas9-based gene editing.

It emphasizes the importance of understanding these pathways for performing precise and efficient genetic modifications. While NHEJ is an error-prone repair pathway, suitable for inducing INDELs and generating gene knockouts, HDR offers precise repair using homologous sequences.?

Our experts can select between these pathways depending on the goal of our clients to assure the optimal gene editing strategy.

Read the full article here.


Advancing Developmental Neurotoxicity (DNT) Assessment

At ZeClinics, we're not just participating; we're pioneering the future of developmental neurotoxicity (DNT) assessment. The OECD is leading the charge in exploring innovative methodologies that set new standards in regulatory compliance.

Through collaboration with esteemed partners such as the European Food Safety Authority (EFSA) and USEPA , we're driving the advancement of alternative methodologies for regulation. Our dedication to cutting-edge non-animal models, like zebrafish, not only ensures more accurate and predictive outcomes but also revolutionizes the way we approach regulatory science.

Curious to learn more about how our groundbreaking DNT assessment services can benefit your organization? Connect with us today for further information and discover the future of regulatory compliance.

Claudia Miguel and Valentina Schiavone at DNT5 and OECD Experts Meeting in Konstanz.

Meet Us at CRISPR MED Next Week!

Are you currently seeking innovative methods to accelerate and secure your pipeline?

We specialize in ensuring your success at every phase of early drug discovery, using the power of zebrafish as a clinically relevant, whole organism model.?

This approach enables you to:

  • Gain in vivo functional insights
  • Enhance repurposing and precision medicine initiatives
  • Minimize clinical attrition by selecting your most promising compounds

At ZeClinics, we hold an official CRISPR/Cas9 license. By combining this technology with high-throughput automated phenotyping, we create customized genetic models for target identification, validation, and drug screening purposes.

Our expertise spans across Cardiovascular, Neurodegeneration, Parkinson's, Genetic epilepsy, and Ophthalmology, contributing to numerous global projects.

Curious to see how we can tailor your screening platforms to advance your pipelines? Let's schedule a chat! @Dani and @Flavia are keen to arrange a meeting during the upcoming CRISPR MED24 congress or connect at booth B-3-S during the event.

Feel free to contact us here.


Featured service

What?

A zebrafish-based platform for fast disease modeling and target screening.

How?

By phenotypic characterization of F0 mutant or transgenic larvae.

  1. Generation of F0 knockout & TRANSGENIC models: To quickly infer genetic function in injected fish, bypassing the months-long process of establishing an isogenic mutant line.
  2. High-throughput phenotyping (morphology, functionality, behavior): For fast target validation & rapid identification of hits able to reverse the mutation-induced phenotypes.


Do you have any questions or want to discuss your project plans?

Our experts are ready to help you!

Contact us here!

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