In chromatography experiments, the insert tube plays an important role in HPLC and GC analysis. The correct application of the insert tube (Insert) can help improve the accuracy and sensitivity of chromatographic analysis. The following are some methods of correctly applying the insert tube and Precautions:
- Choose the right insert: Choose the right insert based on your sample type, analytical needs, and column specifications. Inner tubes are usually available in different diameters, lengths, and materials. The most suitable inner tube should be selected according to the experimental conditions.
- Prepare the inner cannula: Before using the inner cannula, make sure that the inner cannula is clean, dry, and free of dirt or residue. The inner tube can be cleaned with detergent and pure water and then dried with airflow or a dryer.
- Filling the sample: Fill the prepared sample into the inner insert tube with a pipette and other tools to ensure that the sample is evenly filled and no bubbles are generated. Avoid sample spillage or contamination of the outside of the inner cannula.
- Install the inner insert tube: Gently insert the inner insert tube containing the sample into the chromatographic column's inlet, ensuring that the inner insert tube and the injector are tightly connected to avoid leakage or misalignment.
- Operation process: According to the requirements of the experimental method and equipment operation manual, set the injection parameters, such as injection volume, injection speed, etc. During the injection process, ensure that the inner insert tube does not move or shake to not affect the analysis results.
- Proper storage: After use, clean the inner tube in time, dry and store it. The inner cannula should be stored in a dry, clean, and dark environment to avoid contamination and oxidation.
- Regular inspection and replacement: Regularly check the status of the inner tube. If dirt, damage, or aging is found, promptly replace the inner tube with a new one to ensure the accuracy and reliability of analysis.
In general, the correct application of the inner cannula requires attention to the selection of the appropriate inner cannula, sample preparation and filling, installation and operation details, and regular inspection and maintenance of the inner cannula, to ensure the accuracy and reliability of the chromatographic analysis.
