PROTEOMICS AND MASS SPECTROMETRY FACILITY
CEINGE Official Profile - Biotecnologie Avanzate Franco Salvatore
Molecular Biology - Advanced Biotechnology - Research and Diagnostics of Genetic Diseases - High-tech Facilities
OUR SERVICES
The CEINGE Proteomics and Mass Spectrometry Facility provides a series of highly technological analytical services aimed at the identification, quantification and structural characterization of peptides, proteins and biomolecules in general.
All analytical services include:
? specific consultancy
? interpretation of results and drafting of reports (on request)
? data retention for three years
? conversion of data into a format suitable for publication
? specific agreements for multiple analyses or for large numbers of samples
The analytical procedures are ISO2001:2015 certified
Contact: Prof. Maria Monti [email protected]; phone 0039 0813737919
Dr. Simona Celentano [email protected]; phone 0093 0813737895
MASS SPECTROMETRY SERVICES
-Determination of the molecular weight of proteins and peptides by ESI-MS and/or MALDI-MS techniques
-Peptide mapping for primary structure analysis of recombinant proteins, protein variants, post-translational modifications, assignment of disulphide bridges
-Sequencing of peptides by Tandem Mass Spectrometry
-Glycoprotein analysis: glycoform profile by MALDI-MS-Oligonucleotide analysis
PROTEOMICS SERVICES
-Identification of proteins from?gel and in solution by LC-MS/MS of purified and/or complexed samples
--Label Free Differential analysis, including statistical analysis and functional clustering with programs such as String, Cytoscape etc. (on request)
-Analysis of post-translational modifications
-Absolute and relative quantification of target proteins in complex matrices by tandem mass spectrometry methods (MRM type)
INTERACTOMICS SERVICES
-Interactomics analysis with purification of protein complexes by immunoprecipitation and protein identification
-Analysis of protein-ligand interaction, using the SPR technique with the definition of kinetic and thermodynamic constants of the interaction
MALDI TOF-TOF 4800 PLUS
LC-MSMS QTOF 6530 System (Agilent)
LC-MSMS XEVO TQS System (Waters)
LC-MSMS 5500 QTRAP System (AB Sciex)
LC-MSMS LTQ Orbitrap XL System (ThermoFisher)
LC-MSMS Exploris240 System (ThermoFisher)
BIACORE X-100 (CYVITA)
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