Expansion microscopy for the analysis of centrioles and cilia
Volodymyr Nechyporuk-Zloy
10%+ Growth Driver | Manager | Microscopist | DL Imaging
Expansion microscopy for the analysis of centrioles and cilia
N. SAHABANDU, D. KONG, V. MAGIDSON, R. NANJUNDAPPA, C. ULLENBERGER, M. MAHJOUB, Jadranka Loncarek
https://doi.org/10.1111/jmi.12841
?Centrioles are vital cellular structures that organize centrosomes and cilia. Due to their sub‐resolutional size, centriole ultrastructural features have been traditionally analyzed by electron microscopy. Here we present an adaptation of magnified analysis of the proteome expansion microscopy method, to be used for a robust analysis of centriole number, duplication status, length, structural abnormalities and ciliation by conventional optical microscopes. The method allows the analysis of centriole's structural features from large populations of adherent and nonadherent cells and multiciliated cultures. We validate the method using EM and super‐resolution microscopy and show that it can be used as an affordable and reliable alternative to electron microscopy in the analysis of centrioles and cilia in various cell cultures.