Holotomography Application - 3D Embryo Assessment
TOMOCUBE, INC.
See your samples like never before with Tomocube’s revolutionary label-free, live cell Holotomography
Explore a revolutionary application of Holotomography in embryogenesis research, highlighting its ability to provide detailed, noninvasive 3D imaging for enhanced assessment and prediction of embryo development in IVF.
Label-free 3D Morpho-Kinetic Profiling for Embryo Quality Prediction
In a recent study, researchers developed a groundbreaking noninvasive technique combining 3D imaging and machine learning to enhance the prediction of embryo quality and blastocyst formation in in vitro fertilization (IVF).
Key findings:
- Low-coherence Holotomography provided detailed, noninvasive 3D visualization of the embryo development from the 2-cell stage to the blastocyst over a span of 72 hours.
- Key 3D morpho-kinetic parameters serves as novel, noninvasive markers for evaluating embryo quality at different developmental stages.
- Multi-nucleation, blastomere evenness, cytoplasmic homogeneity, and fragmentation rate efficiently predict blastocyst formation potential at the cleavage stage.
- A decision tree ensemble model utilizing Holotomography 3D morphological parameters can predict developmental potential with up to 95% accuracy.
Holotomography Platform for Developmental Biology
Features
- Long working distance for high-resolution imaging of thick embryo specimens
- Low-intensity illumination for safe observation without compromising image quality
- Build-in incubator for long-term monitoring of embryogenesis process
[Video] High-resolution 3D Visualization of Mouse Embryo Development