- The particle size of the chromatographic packing mainly affects the column efficiency and back pressure of the packed column. The smaller the particle size of the packing, the higher the column efficiency of the packed column. Under the same selectivity conditions, increasing column efficiency can improve resolution. The smaller the particle size of the filler, the higher the column pressure. Therefore, the requirements for the chromatography system are higher, the investment in equipment is greater, and the price of small particle size chromatography fillers is also high.
- Therefore, the particle size of chromatography fillers used for industrial preparation is often above 10 microns, the particle size of silica gel packing for conventional HPLC columns is between 3-5 microns, and the chromatography fillers used for UPLC are below 2 microns. The selection of chromatographic particle size should take into account separation efficiency and cost.
- In addition to the particle size, the particle size distribution of chromatographic fillers is also an important parameter for the performance of chromatographic fillers. In the high performance liquid chromatography separation process, the channels through which the mobile phase flows are mainly the gaps between the granular fillers, and the inhomogeneity of the filler particle size, uniformity, and tightness of the packed column bed will cause the packed column to produce a multipath effect. . The flow path and retention time of solute molecules in the packed column bed are changed, resulting in broadening of the chromatographic peaks, thus affecting the separation effect of solute molecules.
- When the particle size distribution of chromatographic microsphere packing is wide, when the same solute molecules flow through the chromatographic column, different pathways are generated in the packed column bed, resulting in different path lengths and corresponding retention times, which broadens the chromatographic peaks. , the number of theoretical plates becomes smaller and the column efficiency decreases.
- When microsphere packing with uniform particle size is used to fill the chromatographic column, its tightness is consistent, which effectively reduces the multi-path effect of the packed bed, so that the path length experienced by the solute molecules when flowing through the chromatographic column is basically the same, and the corresponding retention time is also It is more consistent, narrows the chromatographic peak width, and increases the number of theoretical plates, thereby obtaining higher column efficiency and achieving better separation results in reversed-phase separation of small molecules and polypeptides.#LiquidChromatographyTips?#PumpMaintenance?#InjectorCare?#AccurateAnalysis?#LaboratoryTechniques?#Column?#CrimpTopVials?#AutosamplerVials?#GCAnalysis?#HPLCAnalysis?#SampleIntegrity?#Efficiency?#Reliability?#ChemicalResistance?#LiquidChromatography?#SampleHandling
