Edition 23: April 2024
Next Generation Sequencing: An NGS Overview & Update
Next Generation Sequencing (NGS) is a modern sequencing technology also known as high throughput sequencing. It allows RNA/DNA sequencing to be carried out much more quickly than Sanger sequencing. NGS includes Illumina, Roche 454, Ion Torrent, and Solid sequencing.
New Applications of Microsampling Technology in NGS
Microsampling devices like the Neoteryx? Mitra? device based on VAMS? technology by Trajan Scientific and Medical can allow for the production of accurate results from molecular research labs. Finger-stick blood collection via remote microsampling is known for being minimally invasive, less costly than other blood collection methods, and very efficient in both clinical and remote research settings. Several research groups have recently applied Mitra microsampling in omics studies.
Metabolon Validates a Method for Metabolomic Profiling in Blood Microsamples
This year, Metabolon used Mitra? devices to validate a biological, analytical method on their Global Discovery Panel for metabolomics studies.
Analytical validation ensures that the blood microsamples maintain sensitivity, precision, and stability throughout the sample journey, from collection by the study subject to processing and analysis by researchers in the lab. Biological validation confirms that known signatures in?other?blood samples?will be detectable in a reproducible way.
Metabolon’s validation of Mitra with VAMS revealed reproducible results within an acceptable relative standard deviation (RSD). For the Metabolon Global Discovery Panel, 20 μL sample volume is optimal to identify the most metabolites using the smallest amount of sample. More than 90% of metabolites assessed had good precision between four technical replicates.
For details on the work by Metabolon and other research groups, read our blog: A Brief Introduction to Next Generation Sequencing (neoteryx.com)
To learn more about the Metabolon study, visit their web page on microsampling for metabolomics.
President at GenTegra LLC
10 个月Its great see more and more users recognizing the value of the combination of GenTegra LLC Active Chemical Protection room temperature stabilization technology with the Neoteryx Mitra? device for RNA research. The ability to stabilize and extract high integrity RNA at room temperature from remotely collected whole blood microsamples is game changing. Validation of GenTegra LLC room temperature stabilized RNA from Neoteryx Mitra? devices on the Promega Corporation Maxwell System takes it to the next level. #rna #NGS #microsampling #transcriptomics