EAS HPLC courses for pharm analysis: Nov 12-13, 2023.

EAS HPLC courses for pharm analysis: Nov 12-13, 2023.

I will offer a 2-day, all-inclusive, intermediate-level HPLC course for pharm analysis this November at EAS. Registration link is: https://eas.org/5072-2-2/

EAS will provide breakfast, lunch, and two coffee breaks daily. These courses provide an excellent value for those interested to upgrade their knowledge in HPLC in pharm analysis and other applications areas. Here is the course agenda.

HPLC and UHPLC for Practicing Scientists 1 and 2: Fundamentals, Method Development, and Troubleshooting

Dr. Michael W. Dong, Norwalk, CT, USA (2-day), [email protected]

?Course description

This intermediate, all-inclusive, 2-day short course will provide the analytical scientist with a clearer understanding and a solid working knowledge of the concepts, instrumentation, columns, applications, and best practices in method development and troubleshooting of HPLC and UHPLC (ultra-high-pressure liquid chromatography). The focus is on the pharmaceutical analysis of small-molecule drugs.?

Who Should Attend?

Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want to get an updated overview of the fundamentals of HPLC and UHPLC in pharmaceutical analysis and other applications. A basic understanding of chemistry and HPLC with some hands-on experience is assumed.

Day 1: HPLC 1: Fundamentals

1. Concepts ?

A.??? Introduction:? The chromatographic process, advantages and limitations, and chromatographic modes (NPC, RPC, IEC, SEC)

B.??? Concepts: Retention factor (k), separation factor (a), column efficiency (N), resolution (Rs), tailing factor (Tf), the resolution equation, the linear solvent strength theory, and column void volume (VM)?

C.?? Mobile phase factors (organic modifiers, pH, buffers), mass spec compatibility, isocratic and gradient separations, operating parameters (Flow, gradient time (tG), column temperature (T)), peak capacity (Pc)?

2.??? HPLC Columns, Trends, and Selection Guides ? ?

A.??? Column fundamentals: Glossary, Characteristics, and types, packing characteristics (support type, particle size, pore size), bonded phases, end-fittings, plate height (H), the van Deemter equation.

B.??? Trends: Trends of shorter and smaller columns and the use of high-purity silica, traditional and novel bonding chemistries, the concept of “orthogonality,” listing of common RPC columns, column selection guide?

C.?? Newer column types: Hybrids, charged surface hybrids, HILIC, sub-3 and sub-2 mm, and superficially porous particles (SPP)

?

1. HPLC Instrumentation and Operating Principles ? ?

A.??? The system, Pump, and Autosampler. Integrated vs. modular system, solvent delivery system, low-pressure vs. high-pressure mixing, dwell volume, pump trends, autosampler types, and trends. ?

B.??? Detectors: Operating principles of UV/Vis detection and characteristics, photodiode array detector (DAD), newer detectors for non-chromophoric analytes (ELSD, CAD).

C.?? MS, CDS, and Instrument Bandwidth. The mass spectrometer (MS), types and ESI interface, Data Chromatography System (CDS), instrumental bandwidth

Day 2: HPLC 2: Applications, UHPLC, Method Development, and Troubleshooting

4.??? HPLC Applications and an Overview of UHPLC

1. HPLC applications include pharmaceuticals, biopharmaceuticals, food, environmental, chemical, and bio-separations.
2. UHPLC overview, perspectives, and benefits: UHPLC concepts, instrumentation, and benefits (fast separations, high-resolution analysis, and rapid method development)
3. Potential issues and how to mitigate (viscous heating, operating nuances, compatibility to existing methods, injection precision, detector sensitivity vs. mixing volumes), method translation from HPLC to UHPLC, and transition from HPLC to UHPLC.?

5.??? HPLC Method Development and Validation ? ?????????

A.??? Tradition strategy for HPLC method development and a survey of automated tools and software

B.??? 3-pronged template approach for rapid method development

C.?? Use of a universal generic gradient method for the assay of multiple NCEs and stability-indicating assays with simple adjustments

D.?? A brief overview of method validation with case studies.?

6.??? HPLC Operation and Troubleshooting

A.??? Mobile phase and sample preparation and best practice in HPLC operation ?

B.??? Maintenance and troubleshooting guide with case studies

C.?? Diagnosing and solving problems (pressure, baseline, peak, data performance)

?

Biography

Dr. Michael W. Dong is a principal consultant in MWD Consulting, focusing on consulting and training services on HPLC, pharmaceutical analysis, and drug quality. He was formerly a Senior Scientist in Analytical Chemistry and Quality Control at Genentech, Research Director at Synomics Pharma, Research Fellow at Purdue Pharma, Senior Staff Scientist at Applied Biosystems/Perkin-Elmer, and section head at Celanese Research Company, and postdoctoral research fellow at Naylor-Dana Institute for Disease Prevention.

He holds a Ph.D. in Analytical Chemistry from the City University of New York and a certificate in Biotechnology from the University of California Santa Cruz. He has 130+ publications and five books, including a bestselling book on chromatography (HPLC and UHPLC for Practicing Scientists, 2nd Ed., Wiley, 2019). He is an advisory board member of LCGC magazine and the Chinese American Chromatography Association. He has been a “Perspectives of Modern HPLC” columnist for LCGC North America since 2013.

LinkedIn profile: https://www.dhirubhai.net/in/michael-dong-%E5%94%90%E7%BA%AC%E4%B8%AD-8390548/

MWD Consulting website: https://www.mwd-consulting.com/

Your connection to LinkedIn is welcomed, and many HPLC and pharm analysis resources, including 30+ white papers, are available for free download on my website.

Recommended textbook:

M. W. Dong, HPLC and UHPLC for Practicing Scientists, 2nd Ed., Wiley, Hoboken, New Jersey, 2019. The list price is $103, though it can be purchased at Amazon.com at a substantial discount for either the paperback or the E-book ($72 for paperback). While the short course provides a quick overview of key concepts and practices of HPLC, the book provides a systematic and complementary, reference guide.