Discover the Power of Two-step Allelic Exchange
1. A Guide To Leveraging Single-Cell CRISPR Screens for Deeper Biological Insights
Explore the transformative potential of single cell CRISPR screening, where you'll learn how to profile hundreds of CRISPR perturbations and uncover mechanistic insights into development, disease, and therapeutic responses using advanced gene expression technologies.
In this webinar, you will learn:
? Tips and best practices for success for CRISPR experimental set-up from delivery to selection
? Key considerations for single-cell CRISPR screening from library preparation to sequencing
? Data analysis guidance for leveraging single cell CRISPR screening data for mechanistic insights into gene function
With MilliporeSigma
2. Old Reliable: Two-Step Allelic Exchange
Two-step allelic exchange is a precise and reliable method for gene manipulation. In this article, learn how suicide vectors enable targeted genetic alterations in bacteria, enhancing our understanding of gene function and expression with step-by-step guidance on implementing this technique effectively.
3. To Boil? Or Be Boiled? Saving Time With Colony PCR
Ever wondered:
? Did my electroporation work?
? Is my vector inside my competent cell?
? Do I have contamination in my liquid culture?
These are just a few of the questions you can answer using colony PCR. With this cheap and easy PCR technique, you directly amplify the entire colony, bypassing the conventional DNA extraction process! Learn more in this article.
-Thomas Warwick, Content Creator, Bitesize Bio