Design and development of a two-color emissive FRET pair based on a photostable fluorescent deoxyuridine donor presenting a mega-Stokes shift

Design and development of a two-color emissive FRET pair based on a photostable fluorescent deoxyuridine donor presenting a mega-Stokes shift

Abstract

We report the synthesis and site-specific incorporation in oligodeoxynucleotides (ODNs) of an emissive deoxyuridine analog electronically conjugated on its C5-position with a 3-methoxychromone moiety acting as a fluorophore. When incorporated in ODNs, this fluorescent deoxyuridine analog exhibits remarkable photostability and good quantum yield. This deoxyuridine analog also displays a mega-Stokes shift, which allows its use as an efficient donor for FRET-based studies when paired with the yellow emissive ?indocarbocyanine Cy3 acceptor