MAbs for COVID-19 Etiological and Serological Examination

Monoclonal Antibodies for Development of COVID-19 Serology Assays 

To detect and measure the levels of SARS-CoV-2 antibodies as a result of exposure to COVID-19 will provide valuable information on the development of individual immunity. COVID-19 specific IgM antibodies can be detected after 3-5 days from onset of the disease. IgG antibodies appear later, however, their titer is 4 times higher in the recovery period than that in the acute phase.

While IgA has gained little attention by developers of SARS-Cov-2 antibody tests, recent publications suggest that including it in serology assays could improve the sensitivity of the tests (Yu et al., 2020; Ma et al., 2020). The data obtained in these studies indicate that IgA antibodies can be detected earlier than IgM or IgG antibodies. Also, IgA (and IgG) antibodies seem to positively correlate with the severity of the disease.

At Advanced ImmunoChemical, we provide several antibodies for the development of serology assays:

Anti-IgG, in vitro, antibody. Most popular: MAb 2B9cc

Anti-IgM, in vitro, antibody Most popular: MAb 5A9cc

Anti-IgA, in vitro, antibody

Our Anti-IgM and Anti-IgG can be used as secondary antibodies in serology assays detecting SARS-Cov-2 (Covid-19). The principle is as follows:

SARS-Cov-2 antigen, e.g. recombinant spike S1 antigen, is bound to a plate. If the patient has been infected with COVID-19 in the past, they will have developed antibodies against the virus. From their serum sample, these SARS-Cov-2 specific antibodies bind to the recombinant antigen. Our anti-IgG/IgM antibodies will then bind to the SARS-Cov-2 antibodies. If the SARS-Cov-2 antibodies are not present in the patient serum sample, then there is nothing to bind to the antigen and nothing for our anti-IgG/IgM antibodies to bind with.

Major Predictors of COVID-19 Severity

We would like to remind you that testing for SARS-CoV-2 is not the only requirement in regard to managing COVID-19. Screening and monitoring for the various health issues that may arise as part of COVID-19 disease management are also needed. You can find the following markers in our portfolio as major predictors of COVID-19 severity:

Troponin I, cardiac

Troponin T, cardiac

CRP

Cystatin C

D-dimer

Myoglobin

NT-proBNP

Procalcitonin

SAA

Advanced ImmunoChemical is open and business continues as normal.

We would like to confirm that we are continuing to supply our products as normal and do not foresee any significant disruption to our product availability or shipping schedules. However, circumstances can change rapidly. Therefore, we will continue to monitor developments and do our best to let you know as soon as possible if the situation regarding our product supply changes.

?References:

1. Giuseppe Lippi (2020) Coronavirus Disease 2019 Video. Pearl of Laboratory Medicine. Clinical Chemistry Trainee Council, AACC.

2. He XW, Lai JS, Cheng J, et al. Zhonghua Xin Xue Guan Bing Za Zhi. (2020) ;48(0):E011. doi:10.3760/cma.j.cn112148-20200228-00137

3. Lippi G, Lavie CJ, Sanchis-Gomar F. Cardiac troponin I in patients with coronavirus disease 2019 (COVID-19): Evidence from a meta-analysis [published online ahead of print, 2020 Mar 10]. Prog Cardiovasc Dis. 2020;S0033-0620(20)30055-4. doi:10.1016/j.pcad.2020.03.001

4. Zhou F et al. (2020) Clinical Course and Risk Factors for Mortality of Adult Inpatients With COVID-19 in Wuhan, China: A Retrospective Cohort Study. Lancet 2020;Mar 9:[Epub ahead of print].

5. Jianlin Xiang et al. (2020) Potential biochemical markers to identify severe cases among COVID-19 patients. medRxiv 2020.03.19.20034447; doi: https://doi.org/10.1101/2020.03.19.20034447

6. Chinese Clinical Guideline for COVID-19 Pneumonia Diagnosis and Treatment (7th edition)

7. Yu, H. et al. Distinct features of SARS-CoV-2-specific IgA response in COVID-19 patients. Eur Respir J 2001526 (2020) doi:10.1183/13993003.01526-2020.

8. Ma, H. et al. COVID-19 diagnosis and study of serum SARS-CoV-2 specific IgA, IgM and IgG by chemiluminescence immunoanalysis. https://medrxiv.org/lookup/doi/10.1101/2020.04.17.20064907 (2020) doi:10.1101/2020.04.17.20064907.

Browse our catalog and order online at: www.advimmuno.com/shop

Best regards,

Anne Tolles, M.Sc./Pres.