Cas9 mRNA Separation on SEC-MALS
Genome editing is one of the most exciting new areas of therapeutic development, in which Cas9 mRNA genome editing, often referred to as CRISPR-Cas9 technology, is considered one of the most promising developments. In this study, we present SEC HPLC data to analyze the effect of pore size, surface chemistry, type of buffer system, salt concentration and temperature on the aggregate separation of commercially available Cas9 mRNA by using both UV and MALS detections.?
Sepax SRT? SEC 1000 ? and 2000 ? columns outperform competitor's?1000 ? SEC column under all buffer systems, with significantly higher resolution. Sepax 2000 ? SEC column gives the highest resolution among the three columns being screened, which demonstrates a larger pore size is more suitable for the Cas9 mRNA, with 4522 nucleotides size. Sepax SRT SEC surface chemistry also provides unique selectivity and universal buffer compatibility with 50% lower pressure, which is important for developing a robust, accurate, and reliable analytical method.
Cas9 mRNA (4522nt) SEC UV-MALS - Tris + NaCl Buffer
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