Cas9 or Cas12a? Benefits of Expanding Your Gene Editing Toolbox
In our latest post, we focus on the differences and advantages of Cas12a over Cas9 in gene editing. Cas12a has a unique ability to edit AT-rich genomes and create uniform DNA lesions, which are beneficial for homology-directed repair. This feature is especially suitable for multiplex gene editing and increases its potential in gene therapy and diagnostics. By comparing it with the well-established Cas9, the article illustrates how Cas12a offers complementary and expanded capabilities in genome editing, providing researchers with more versatile tools in the field. Read the full blog post HERE
Modeling Anxiety And Stress In Zebrafish: Thigmotaxis
Thigmotaxis is a “wall hugging” behavior exhibited in both zebrafish and mice. Thigmotaxis has been used to model anxiety and stress response - this said, Thigmotaxis assays, both in adult and larval zebrafish, offer researchers powerful tools to explore anxiety mechanisms and drug responses with efficiency and ethical considerations in mind.?
Zebrafish Genome Editing: CRISPR Vs. Tol2
At InVivo Biosystems, we specialize in multiple different ways of zebrafish genome editing?such as an insertion, deletion, or replacement of DNA at a specific site in a genome to achieve?site-directed mutagenesis,?knock-in tagging, and?Tol2 transgenesis. These all allow us use the zebrafish to study detailed characteristics of its human ortholog, but what are the differences, and how do you know what to use when?
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As a researcher, why choose Zebrafish for Studying Host-Microbe Interactions?
In our recent blog post, Joseph Bruckner, Ph.D. shared his expertise on why and how zebrafish have become the gold standard model for studying host-microbe interactions. Bruckner utilized zebrafish as a cost-effective and scalable model for his research correlating gut microbiota with forebrain neuronal circuitry.Judith Eisen, Ph.D., a pioneer and leader in zebrafish innovation recently gave a keynote lecture on how host-associated microbes modulate neural genetic expression titled "From Guts to Brains" at the International Zebrafish Society's annual Strategic Conference of Zebrafish Investigators. Eisen has been honored in the Academy of Arts and Sciences for her neuroscience research contributions and has been a long-time supporter of InVivo Biosystems. She also happened to be Joseph Bruckner's Principal Investigator during his post-doctoral research at the University of Oregon. See the full blog HERE