Brighten Up Your Images in Multiphoton Microscopy??

To improve the contrast and brightness of your nonlinear fluorescence images, TOPTICA’s FemtoFiber ultra 920 incorporates Clean Pulse Technology - a laser design that significantly reduces pedestals and side-wings on the temporal pulse profile compared to other laser amplifier designs.?

For the excitation laser in 2-photon microscopy, a combination of high temporal pulse quality (a “clean pulse”) and a short pulse duration at the sample plane improves the contrast and brightness of fluorescence images.?

In order to achieve the best image quality, TOPTICA’s FemtoFiber ultra is equipped with Clean-Pulse Technology that reduces pedestals and side-wings on the temporal pulse profile. Hence, the full laser power contributes to two-photon excitation. Clean-Pulse Technology provides highest fluorescence image brightness and contrast while keeping thermal heating of the sample to a minimum.

Additionally, TOPTICA’s FemtoFiber ultra provides motorized group delay dispersion (GDD) pre-compensation that can be controlled in software.

Examples of Two-photon microscopy imaging without and with Clean Pulse Technology

Femtosecond fiber lasers for simplifying microscopy

TOPTICA’s femtosecond fiber lasers provide optimal performance while being easy-to-use. In particular for neuroscience applications, TOPTICA’s FemtoFiber ultra lasers are ideally suited to address biological questions by imaging green (GFP) and red fluorescent proteins (RFP), and are already widely adopted in non-linear microscopes.

FemtoFiber ultra 920?

• Brighter images with Clean Pulse technology
• Fully turn-key with integrated AOM and GDD
• No noise-stress for animals thanks to fan-free passive cooling
• Compact laser design saving valuable table space
• Low cost of ownership using robust & reliable fiber-laser technology

TOPTICA’s Clean-Pulse Technology

TOPTICA offers laser solutions? for a multitude of biophotonics applications:?

• 2-Photon Microscopy
• Multiphoton Microscopy
• Confocal Microscopy
• Raman Spectroscopy and Microscopy
• Diffuse Correlation Spectroscopy (DCS)
• Flow Cytometry
• Fluorescence Detection and Imaging
• SHG Microscopy
• High Content Screening (HCS)
• FLIM, FRET, FRAP etc.
• Wide Field Imaging Techniques (e.g. TIRF)
• Super Resolution Imaging including STED, PALM, STORM
• Medical Imaging (e.g. Fluorescin Angiography)
• Microplate Readers
• High Throughput Screening (HTS)
• DNA Gene Sequencing

Visit www.toptica.com to learn more about our lasers for biophotonics & neuroscience