Boosting Higher Purity of mRNA | The Best Combo of Synthetic Modulation & Detection Tools for Minimized dsRNA Contamination

In addition to the core delivery technology, the development of mRNA vaccines and drugs also needs to focus on the purity of mRNA synthesized in vitro. dsRNA impurities generated during mRNA synthesis can stimulate RIG-I and MDA5, etc., triggering downstream antiviral innate immune signaling pathways and causing immune responses. As a therapeutic drug, the quality control process of mRNA synthesis and purification is very important because of the high dose and high frequency of administration, if the impurity quality control problem occurs, the patient may experience strong adverse reactions.

WorkFlow

Why choose our solution with low dsRNA for transcription synthesis of mRNAs?

T7 Turbo RNA Polymerase(200 U/μl, GMP Grade)：mRNA development with less dsRNA.

Reduce dsRNA content more than 5-fold

T7wt?and?T7?Turbo?RNA?Polymerase?were?co-transcribed?respectively?and?purified?by?magnetic?beads,?and?the?dsRNA?content?was?determined?by?Vazyme?Elisa?kit.?The?results?showed?that?T7?Turbo?RNA?Polymerase?could?reduce?the?dsRNA?production?by?about?5?folds?for?different?length?sequences?and?modification?types.

EasyAna dsRNA (Modified) Quantitative Detection Kit (ELISA)2.0：A more sensitive and accurate dsRNA detection tool for >40 bp dsRNA.

Validated by rigorous methodology

• A lower limit of quantification to support low dsRNA process development assays.

The?common?dsRNA?detection?methods?were?validated.?The?results?showed?that?dot?blot?assay?was?semi-quantitative,?and?the?precision?and?other?indicators?could?not?be?compared?with?ELISA.?Compared?with?supplier?A?under?the?same?Elisa?method,?Vazyme?kit?has?a?higher?lower?limit?of?quantitation?and?precision.

• Provides more accurate quantification and consistent response to dsRNAs of different lengths and modifications.

Supplier?A?and?Vazyme?ELISA?kits?were?used?to?recycle?dsRNA?standard??synthesized?by?different?sequences&NTP?with?different?modifications.?The?results?showed?that?the?Vazyme?kit?could?accurately?quantify?different?sequences?and?different?modified?dsRNA.

Learn more information or place order online, please visit >>

https://www.vazymeglobal.com/product-center/mrna-gmp-enzymes/t7-turbo-rna-polymerase-200u-ul-gmp-grade

https://www.vazymeglobal.com/product-center/mrna-qc-reagents/easyana-dsrna-modified-quantitative-detection-kit-elisa-2-0

*All rights reserved. All the statistics comes from Vazyme laboratory.