Bond vs. Dr. Salmonella

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Miss Pennymoney: "Jaaaames, you look terrible.

What did you do last night?

Never mind, I can well imagine.

You're tardy, Mr. Bond.

They're expecting you over an hour ago."

Bond: "Miss Pennymoney, might you have an instant BloodyMary about you?

My stomach feels like it's been hit by a freight train From Russia with (no) Love (Putin, - depicted below).

Wait! The bathroom," as he rushes towards the door, "I'll be back!"

When Bond returns to the office, Ms. Pennymoney ask James - - - .

Miss Pennymoney: "What did you have to eat last night, James?

You won't get a Quantum of Solace from the Boss on this morning, she is rather short tempered."

Bond: "Stuffed chicken breast, why?"

Miss Pennymoney: "Really, Mr. Bond?

Had you not heard on Royal CNN - Great Britain's Special Edition, there's a salmonella outbreak associated with stuff chicken breast across all of Europa?

I've never imagined such a thing happening."

Bond: "Never say never again, Miss Pennymoney, I was alone last night.

I consumed a TV dinner while watching the tele (Johnny English, cracks me up) with Friskey.

You know, you only live twice, unless - - - you're a kitty Cat."

James, I thought for sure you were out to Sir Paul McCartney's popular "Town Bar and A. Broccoli Grill," certainly not at home alone eating a stuffed chicken nRTE TV dinner with your Friskey.

You really are ill, or perhaps getting an oddment older, aren't you Jaa-mes?"

A buzzing sound rings and a red button flashes on Miss Pennymaker's desktop 2-way.

"They are waiting for you Mister Bond. I'll answer right after you step in. You had better go in now before she turns into - "Maggie Hilda Roberts, The Iron Woman."

Ask poor Denis ."

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Bond: Q how in the hell have you been bloody old chap?

Do you have anything in your magical arsenal for an upset stomach and the runs?"

Q: "Mr. Bond, pleaze. As per verbatim - Her Majestv's Secret Service Index Sec. 3. (no.077 j.b.), indelibly states that Diarrhoea is the correct Oxford English term.

Certainly not the term - "the runs" in 21st century London, Great Britain.

Diarrhoea is very common?and usually not a cause for concern.

It's a term used to describe loose stools that are often a symptom of a bowel infection. This can be contracted through contaminated food, water or spread through illness

Think Covid 19, a sheer virulent spinning air-borne neutron-bomb like virus if there ever was one.

Instead of nuking and killing us all at once - Communist China hit us instead with a cloned invisible lab bug - so they may watch the bodies pile up on the modern streets of 21st century civilizations; while watching every country's economy sink back to the a la 1930's depression in a nano-day.

Wow, what a covert plan that one was."

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Boss: "James you're late, again. Where in God's name have you been?

Casino Royale .. . C?te d'Azur, I suppose !?

I have an important assignment for you." As the Boss continues ---

"James, please. Die another day - It's no time to die now - listen and quit making a Spector of yourself.

Look at you!

Listen. Chicken in chlorine and other disinfectants that are utilized to remove harmful bacteria was a practice banned by the European Union (EU) way, way back in 1997 over food safety concerns.

The ban has stopped virtually all imports of USA GMO, no steroids, cage free level 2, non-GMO organic fed, chicken meat which is generally treated by this process.

It's not consuming chlorine in of itself that the EU was worried about, Mr. Bond.

In fact in 2005 the European Food Safety Authority said that "exposure to chlorite residues arising from treated poultry carcasses would be of no safety concern ".

Chlorine-rinsed bagged salads are very common here in the UK and other countries in the EU.?

But the EU believes that over relying on a chlorine rinse at the end of the meat production process could very well be a way of compensating for poor hygiene standards - such as dirty and / or crowded abattoirs."

Bond: "Dirty abattoirs? Fecal upstream = Fecal downstream to ones Royal China placed with a ruler onto a Maple Wood table - 1789 edition - including too - sterling silver knives, forks and spoons. I thought we were a cage free humane country?

God save the Chicks!"

Q: "May I please continue, Mr. Bond?"

Bond holds his aching tummy as he nods - yes.

Q: "Awareness surrounding the use of chlorine as a disinfectant came about as early 1868, when Edward VII was king. Chlorine was found to be a core chemical in curing puerperal fever.

There are several current control methods for?Salmonella?in the processing setting, namely, good hygiene practices and the use of chlorine containing compounds (examples include: chlorine dioxide and sodium chlorite) as well as organic acids such as lactic acid and succinic acid.

Look at that low pH level regarding the acids."

Although chlorine has been shown to have a high efficacy readings, it has also been shown that it places immense selective pressure on?Salmonella?spp. and is not sustainable in the long term if - - - if antimicrobial resistant spp. are to be eschewed.

The control of?Salmonella?in chicken processing plants is an ongoing challenge around the globe - - - especially with the ever burgeoning demand for poultry escalating processing throughputs at lightening line speeds" as Q projects images from his classical 1960s Keystone reel-to-reel onto Scotland "Sir Arthur Ignatius Conan Doyle" Yards office wall - - - due east / south.

Q - continues: "Foodborne outbreaks due to?Salmonella?poses a prominent risk to public health - world wide!

It's well known even by Queen E, the 2nd, that chicken meat is a good reservoir for?Salmonella, as it is important for chicken processing plants to continuously optimize methods in order to truncate the incidence of?Salmonella?on their products, I dare say - - - Mr. Bond .

Current interventional methods Mr. Bond, includes the use of chemical antimicrobials such as chlorine-containing compounds and organic acids.

However, these current methods are decreasing in popularity due to the ever rising rate of?Salmonella?resistance, (of which all pathogens do - mutate and mate), coupled with the challenge of preserving the more natural sensory properties of the chicken meat, along with the increasing stringency of antimicrobial use, I dare say.

Bacteriophages, Mr. Bond, Bacteriophages.

I love these ancient Darwinian driven, God given, pre-cursors to Nano technology!

Anyways, Bacteriophages, also known quite simply as phages, if you so prefer, are actually?viruses that infect and replicate only within bacterial cells.

They are ubiquitous in the environment and are recognized as the most abundant biological agent on earth. Sir Arthur C. Clarke, writer, visionary, out-of-the-box-thinker, could have have written a dandy Sci-Fi back in the day."

Bond: "Is China behind their self-imposed iron curtain once again - or is it that Sick-O Wanna-B Putin again?

American General George Patton was right, we should have occupied Russia at the end of WW 2, those sons-of-a-bitches!"

Q: "Bond, please pay attention, this is Bacteriophages - Mother Nature in of herself.

Forget the Attention Wanter of the Cold blooded War Criminal Murderer - and - those Commies in the far east - back-stabbing to thee core - China's Commie, Oppressive murdering Government - for now.

In 2016, the global livestock environmental assessment model (GLEAM) generated by the Food and Agriculture Organization of the United Nations approximated egg production to be 73 million tons and meat production to be 100 million tons.

These numbers are constantly increasing due to population growth, escalating incomes and urbanization. Demand for poultry is increasing not only in developing countries but also developed countries - even here in Great B., mind you.

The demand is met because chickens are intensively produced; chickens rapidly reach a sufficient size and are then harvested and processed through highly automated systems that allow for rapid or oooh so rapid throughputs.?

The shift from free range farming towards intensive practices has allowed for tremendous growth in the supply of poultry as a protein source. Intensive practices have utilized various breeding techniques, feed manipulation and antibiotic administration to optimize size, growth, and desirable attributes.?

Animal sourced protein provides various micronutrients often referred to as?vitamins and minerals, are vital to healthy development, disease prevention, and wellbeing?that are challenging to acquire in sufficient quantities from plant based proteins, such as vitamins A and B, zinc, iron, and calcium.

Poultry is cheap, Mr. Bond, while concurrently being a high quality source of protein and has very few negative associations, I dare say - and - is therefore often the animal protein of choice in developing countries around the world.

In a study conducted by Zeng et al., where trends in meat consumption were tracked and analyzed by using American Yankee Doodle Dandle adults from 1999 to 2016, it was found that chicken consumption increased from approximately 250 g per week in 2000, to 300 g per week in 2016.

Furthermore, in Kuwait of all places, the average poultry meat consumption per capita from 2004 to 2016 was a whopping 64.4 kg/year - approximately 1.2 kg/week.

Another country showing substantial growth is Brazil;

Bond: "Brazil, hey Q?

There was once A Spy who loved me there seemingly many movies ago. She had a Golden Eye, if there ever was one.

Live and let Die - I suppose."

Q: "Yes, Bond, please - globally, poultry is the second highest in terms of meat consumption and is predicted to increase more rapidly than any other meat type.

This makes poultry a predominant source of food-borne illness.

There are a few pathogens strongly associated with food-borne outbreaks in poultry, one of the most common being - - -?Dr. Salmonella and the up and coming Campylobacter."

Bond: "Dr. Salmonella?"

Q: "Yes, just an insider joke here at our Royal ISO 17025 Certified Lab.

The United States' Centers for Disease Control and Prevention (CDC) facilitates an incredible note-worthy system whereby clinics collect samples of bacteria isolated from ill patients and submit them to public laboratories. The certified / accredited ISO laboratories then identify the subtypes of the samples using pulsed field gel electrophoresis (PFGE).

These subtypes are then made available on a database (PuleNet) which is accessible nationwide to various organizations which can identify sources of illness caused by a common PFGE subtype.

I dig this Moonraker - like - technology!

Bond: "Dig this, Q?"

Q: "Yes, yes, - - - so sake, just a flash back to the 60s past, Mr. Bond."

Q clears his throat as he continues ...

Q: "Furthermore, PulseNet also makes use of whole genome sequencing (WGS) which determines the order of bases (genetic fingerprint) in a DNA sequence in a single laboratory procedure.

WGS supplies even more intricate information to assist in identifying outbreaks: PFGE compares 15–30 bands, whereas WGS identifies millions of bands, making it easier to distinguish if the bacteria are in fact the same.

In 2017, the CDC identified poultry products (turkey and chicken) as the dominant source of?Salmonella?infections resulting in illness.

This is purely James Bond Hollywood material, Mr. Bond.

Trust me."

BOND:?"Interesting Q. Miss Pennymoney," as Bond utilizes Boss' deck intercom, "This is Bond - - - James, may I please have a teacup of your private reserve - the always organic GMO-free tea with a Baby Ruth candy bar? Yes, a Cliff bar, would be adequate as a sub.

Aaah yes, continue will you Q."

Q: "Awareness surrounding the use of chlorine as a disinfectant came about as early as circa 1868, when chlorine was found to be a core chemical in curing puerperal fever.

Around 1988, however, it was discovered that compounds containing chlorine also have oxidative properties.

Yes! There are several different chlorine-containing compounds that are used to kill bacteria and are often a popular choice due to a combination of affordability, easy implementation, and a high efficacy, I may add."

Bond: Speaking once again on the desk intercom, "Miss Pennymoney, is the whistling Dixie tea ready?"

Miss Pennymoney: "I'm adding your clearly labeled 95.007 % pure organic sugar as I speak, Mr. Bond."

Q: "When chlorine is added to H2O , it reacts with the hydrogen and oxygen of the water molecule, resulting in the formation of hydrochloric acid (HCl) and hypochlorous acid (HOCl).

HOCl further undergoes dissociation to form hypochlorite (OCl?) and hydrogen (H+) ions.

Boss, can we please have the services of the Cone of Silence before I continue?"

Boss: "Yes, certainly Q. To protect our Trade Secrets N- deed!"

Q: Both HOCl and OCl??account for the “free chlorine” in a solution and are the main compounds behind the antimicrobial action from the addition of chlorine.

The mode of action of free chlorine can be divided into three steps: first, the free chlorine compounds disrupt the bacterial cell wall, which causes bacterial DNA to leach out of the cell.

After this, the free chlorine proceeds to interact with the cell nucleic material and enzymes which inhibits their normal processes.

Finally, the free chlorine may also interrupt transport and respiratory mechanisms in the cell, which negatively effects the cells overall viability.?

Fascinating Stuff --- James my boy!

What a view to kill - The Living!

Slow release chlorine dioxide (SRCD) is often used in the processing of poultry to decrease?Salmonella?on carcasses.

The use of SRCD as an antimicrobial is necessary because, despite efforts that ensure the number of live birds that have?Salmonella?are low, there is an inevitable spread of?Salmonella?due to the mechanical action of the plucking machine, as well as the damage to innards during the evisceration step.

Bond: "Not good, Q."

Q: "Furthermore, the level of contamination of carcasses that ends up in your local and friendly neighborhood supermarket, is something which is strongly correlated to the amount of cross-contamination which occurs during the processing.

A "dab will do 'ya" with a 10% SRCD carcass rinse used in combination with a 50 ppm chlorine solution in the spin chilling step has shown to reduce?Salmonella?by more than 80%.?

But! Lab stats do not only always correspond to real live chicken harvesting conditions.

Despite often being used in combination during processing, SRCD is preferred over chlorine as chlorine may form carcinogenic chlorinated hydrocarbons in the presence of organic matter.

After all, chlorine is on California's Prop 65 list of naughties, naughties."

Bond: "That's saying something."

Miss Moneymaker: "Excuse me gentleman. Take this first, followed with your tea Mister Bond.

This is the new organic, non-GMO edition of pepto bismol.

And it's only a couple Euro's more when compared to the generic one of years past!

Hell of a seller cause it says - organic - you get it, what will they think of next?"

Miss Moneymaker: "Oh, and Mr. Bond - - - London's royal city health officials are ready for your stool sample.

You just might be suffering from a micro dose of - - - Mr. Salmonella."

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Part 2 Bond hitting the road, finally, to meet up with that omni-present Gram-Negative nemesis - Dr. Salmonella

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Sir Sean Connery?was born?Thomas Connery - (1930 – 2020) - a Scottish and an Academy Award winning actor.

He was the first actor to portray fictional British secret agent?James Bond ?on film , starring in seven?Bond films ?between 1962 and 1983.

Originating the role in?Dr. No , Connery played Bond in six of?Eon Productions ' entries and made his final appearance in?Never Say Never Again .

R.I.P. 007 - From TNTC Admirers.

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