Bio-Rad Reads: Digital PCR Data Quality Edition, Vol. 2
Welcome to the April 2024 issue of Bio-Rad Reads! This month, we're taking another deep dive into digital PCR (dPCR) data quality. Here, we’ll discuss how data quality can vary across different kinds of digital PCR experiments, why it's important to maximize the quality of your data, and how only the most reliable instrumentation can provide the high-quality data necessary to empower and accelerate your scientific endeavors.
Read on for perspectives on these crucial topics, as well as a collection of webinars that offer more in-depth information on using dPCR in both research and biopharma settings.
Bad dPCR data can make it difficult to interpret the results of an experiment. But to spot bad data and develop a solution, you need to know what ideal data should look like first. Some positive signs: good data have consistent amplitudes for positive and negative partitions. There should be clear amplitude separation between positive and negative droplets and tight and consistent amplitudes for both positive and negative partitions. Together, this makes it easier to set thresholds between the two, yielding consistent, reliable, interpretable results.
Check out this video from our Did You Know video series to learn more about how to tell good vs. bad data apart and avoid experimental setups that result in poor data quality.
Precision, absolute quantification, sensitivity, tolerance to PCR inhibitors, and multiplexing capabilities: are some of the benefits of using dPCR that make it such a popular method today. Many dPCR technologies are available that digitally quantify nucleic acid species, but not all of them reliably deliver all the benefits of dPCR. So, which to choose?
When exploring your options, it's important to remember that some technologies are more prone to producing bad data due to issues such as hardware quality or optical instability. This can result in noise and cross-contamination, forcing you to spend extra time and resources weeding through messy results and repeating experiments. In contrast, the robust design of Droplet Digital? PCR (ddPCR?) technology overcomes many of these limitations so that you can be confident in your results after the first run.
To learn more about the potential pitfalls of subpar dPCR technology versus quality assays that can be performed on proven instrumentation, watch this video:
When comparing Bio-Rad’s ddPCR technology to the other dPCR platforms on the market today, one little 'd' can make a big difference. By partitioning template nucleic acid molecules into 20,000 distinct droplets and enabling the measurement of thousands of individual PCR reactions, ddPCR technology delivers high-quality data that is dependable, decipherable, and, most importantly, defendable to enhance the quality of your research. Additionally, ddPCR technology is supported by a complete portfolio of assays, consumables, supermixes, protocols, platforms, and software to help you answer your most pressing scientific questions.
To learn more about the differences between ddPCR vs. other dPCR technologies and how using ddPCR technology can give you an edge, watch this video:
领英推荐
Even if you know about the massive power of dPCR, you may not be tapping into its full potential. In this on-demand webinar, Frank Bizouarn, Market Development Manager of the Digital Biology Group at Bio-Rad, will describe steps you can take to maximize data quality and strategies for maintaining diligence and avoiding pitfalls in a biopharma setting.
The key learning objectives are:
Who should attend?
Biopharma scientists who want to implement dPCR in their biomanufacturing workflows or who already use dPCR but want to improve the sensitivity, precision, and accuracy of their results. All webinar participants can request a certificate of attendance.
To learn more, attend the webinar:?Maximizing Droplet Digital PCR Data Quality for Biopharmaceutical Excellence >
dPCR results can deliver absolute quantification and unparalleled sensitivity, but only if you're using high-quality assays and instrumentation to gather your data. ddPCR technology is time-tested and has enabled thousands of peer-reviewed studies, enabling users to achieve maximum data quality and clear experimental findings.
Want to learn more about maximizing data quality in biopharma research and development? We invite you to join Frank Bizouran in a second webinar where he will describe the characteristics of ideal dPCR data, discuss strategies for maintaining diligence, and explore the robust features of the QX ONE? ddPCR System in supporting biodistribution studies for cell therapy products.
The key learning objectives are:
Who should attend?
Biopharma scientists developing cell therapy products who want to implement dPCR in their biodistribution studies or who already use dPCR but want to improve the sensitivity, precision, and accuracy of their results.
Watch the webinar: Achieving Quality and Avoiding Pitfalls in Biopharmaceutical Research >
Did you know that Bio-Rad’s latest promotion makes ddPCR technology more accessible than ever for every lab and every budget? If you've been waiting for the opportune moment to embrace ddPCR technology, now is the time. Adding a ddPCR platform to your lab workflow gives you and your team access to cutting-edge technology that yields high-quality data and catalyzes groundbreaking discoveries. Do you already have one instrument but find that your lab’s demand exceeds the capacity of a single instrument? Take advantage of our special pricing on the QX200 ddPCR System to double your capabilities and empower your lab.
Learn more and get a free, personalized quote today: Droplet Digital PCR for Every Lab, Every Budget >